A Portable Printer and Scanner Assignment Example | Topics and Well Written Essays - 1000 words

A Portable Printer and Scanner - Assignment Example This innovation is occurring through experimentation with new approaches to market development in emerging markets. It is important to reposition this product attracting a wider customer audience often omitted in advertising. High-tech global consumer positioning works well for special interest products associated with prefessional sphere. Since most mobiel professionasl and students who buy and use high-tech products speak the same language and share the same mindset, marketing communications of JetLux will be informative and emphasize performance-related attributes and features. The target group for the Pentax PocketJet will be devided into two sub-groups: people who work at home; small offices (2-3 people). A portable printer JetLux not only allows this group to save time, but it helps to reduce spending on calls to the office and having to wait for someone print the documents. This allows Pentax: to shift its focus to brand image and price reduction measures. to maintained high-speed growth. establish trustworthiness, confidence, and competence for customers. The Impact of Online and Offline Positioning, and Buyers Respond The aim of the message is to appeal to consumers' mind with unique selling proposition. Online and off line promotion will inform potential customers about a new product. This product represents boundless opportunities for marketing campaign within flexible geographical boundaries. Offline buyers 1. Many customers do not have time to read press or listen to the radio, so in-store demonstrations will be the main tool to attract new customers. 2. JetLux will be promoted through computer and mobile stores, based on the fact that there is a strong link between the printer and PC businesses. 3. This target group will involve people who have to work outside of the office and need a high quality printing a low price. 4. It is expected that some customers will buy spontaneously influenced by product demonstrations and evident product benefits. 5. Some of the buyers will go online searching for detailed information and product features. Online buyers 1. The role of Web site is to inform the market clearly and persuasively about the company and its product characteristics. 2. Most buyers are driven by success and brand prestige and brand loyalty. Online buyers value good technology which allows to

Simple Gift Essay Example for Free

Simple Gift Essay The novel ‘the simple gift’ is written by Steven Herrick. He describes three main characters in this book. This book is about one 16 year old boy called Billy, he run away from his house and meets other main characters, Old Bill and Caitlin. Each of there characters received a gifts. Billy is a 16 year old boy. In Billy’s life his father is an alcoholic and is very mean to him. Billy always wants someone who looks after him and then he can learn how to care. This became true when he runs away from his father and meets Old Bill. Old Bill is like a loving father figure to him. So he considered the friendship with Old Bill as a gift. When he stays in the carriage he felt that this is the home for him and Old Bill is his only family. Old Bill is an alcoholic man. He lost his wife and his daughter Jessie. Which makes him a hopeless man and he started drinking to getaway for the suffering (pg.95~98)†I lifted the glass and downed it in tone ignorant gulp and I called for another as all thoughts of truth and beauty washed from my mind†. Old Bill lives in a carriage. Even though he has his own house with a big backyard but he still doesn’t want to live in the house with no one else but himself living in it, with no one else care about him, even himself. This has changed when he met Billy. Every morning Billy brought him breakfast with a bowl of milk and Weet-Bix (pg.76)† Every morning that kid has woken me with Weet-Bix.†, spent time talking with him, and Billy also tried to stop him from drinking heavily. (pg.77, 136)† This kid’s going to turn me into a health. He brought out the ginger beer I swore and laughed.† Caitlin is nearly 18 year old. She works at McDonald’s as a part time hob, to get out of her house (pg.36~37)†I can leave home and that’s why I work at McDonald’s and mop floors.† She’s rich, she had everything she wants but she needs a lover (pg.88)† I know what I really need and it’s not in my bedroom. And it’s not able to be bought in any damn store†. She wants to have a good time with someone she likes. When she met Billy, she had a romantic love experience. They shared food with each other and when she had a lover, her life was perfect (pg.135)†what can I say, it was like stepping into heaven, no less than perfect†. Steven Herrick describes three main characters. Characters offered each other gifts and their gifts had changed each other’s future and are making their life perfect.

Method Development for Protein Detection

Method Development for Protein Detection Binding-induced and label-free colorimetric method for protein detection based on binding-induced DNA hybridization and DNAzymebased signal amplification INTRODUCTION In diagnosing the early stage of a disease or pathological condition, proteins, especially those associated with cancers, are of great importance because they are the molecular machinery of life.[BB2013-AM-3, 1,2] Enzyme-linked immunosorbent assay (ELISA) is the most commonly used method for protein detection.[PD-3,1,2] Unfortunately, this antibody-based assay requires a long incubation periods and long assay times with the involvement of multiple washing steps.[PD-5,1,2] Additionally, it is faced with the challenges of insufficient sensitivity and limited dynamic range.[PD-4, 5,6,7] As an alternative to the antibody-based assay, aptamer-based assays have gained tremendous attention recently.[PD-4,9-11] Aptamers are single stranded DNA or RNA oligonucleotides selected from random sequence nucleic acid libraries through an in vitro selection process termed systematic evolution of ligands by exponential enrichment (SELEX).[PD-6,4-6] They possess high affinity and good selectivity for s mall molecules, proteins or other targets.[BB-11,20-22] Compared to antibodies, aptamers exhibit obvious advantages including a better stability for long-term storage, a rapider preparation by chemical synthesis in large quantity, and the flexible modification with a variety of functional groups.[PD-4, BB-11,23,24] Some aptamer-based amplified detection assays for protein have been developed in the past two decades, such as the polymerase chain reaction (PCR), rolling circle amplification (RCA), strand displacement amplification (SDA) and ligase chain reaction (LCR). [BB-AM-3] Although these amplified assays greatly enhance the sensitivity of protein detection, they are usually time-consuming and too complicated. Therefore, the amplified detection of protein is still challenging in bioanalytical chemistry.[BB2013-AM-3] Deoxyribozymes (DNAzymes) are artificial nucleic acids, which are isolated from in vitro selection. [DM-5] Similar to traditional protein enzymes, they exhibit high catalytic hydrolytic toward specific substrates, while they possess higher thermal stability that can be denatured and renatured formanycycles without losing catalytic activities.[DM-4] This obvious advantage makes DNAzymes ideal biocatalysts for achieving signal amplification in biological applications.[DM-4] An important development in the DNAzyme field is the discovery of the G-quadruplex DNAzyme.[DM-9,9] The G-quadruplex sequences can associate with a cofactor, hemin, to form peroxidase-mimicking DNAzymes to catalyze the H2O2-mediated oxidation of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to a green-colored product ABTS†¢Ã¢Ë†â€™ or enhance the chemiluminescence of the luminol-H2O2 system.[DM-6,25, DM-5,44] With this main advantage, G-quadruplex DNAzyme has been employed to develop many colorime tric, chemiluminescent or fluorescent sensing platforms for the detection of proteins, DNA and other biomolecules.[DM-9,14,DM-2,34-37] Recently, Willners group reported an enzyme-free amplified detection platform based on the hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme.[AC2012-2] This strategy is quite successful, while the target detection is limited to DNA, and the detection of protein represents another challenge. In this work, taking the advantages of the high selectivity and affinity of aptamers and the HRP-mimicking DNAzyme amplification strategy, we designed a new binding-induced and label-free ultrasensitive colorimetric method for amplified detection of protein. As a proof of principle, human ÃŽ ±-thrombin and its two aptamers, Apt29 and Apt15, are used. In this sensing system, Apt29 and Apt15 are integrated into the proximity probes as recognition elements for the thrombin. These two proximity probes hybridize with each other stably only when both of them bind to the thrombin simultaneously.[BB2013-AM-3] The binding-induced hybridization duplex triggers an autonomous cross-opening of the two functional hairpin structures. And this leads to the formation of a variety of hemin/G-quadruplex DNAzymes. The DNAzymes catalyze the oxidation of ABTS, generating a green colorimetric signal, which can be monitored simply by a spectrophotometer. [DM-3,29,30] This binding-induced and DNAzyme-based s ignal amplified method has a great potential for protein detection. [BB2013-AM-3] In addition, since various recognition elements might be fused, this method can be further extended to sensitive detection of other proteins.[DM-4] EXPERIMENTAL SCETION Materials and Reagents. All DNA oligonucleotides were purchased from Genscript (Jiangsu, China). The oligonucleotides were PAGE-purified and diluted in pH 7.4, 20 mM Tris-HCl buffer solution (containing 100 mM NaCl, 20 mM KCl, and 2 mM MgCl2) to give stock solutions of 100 ÃŽ ¼M. Before use, two hairpin structures were heated to 95à ¢Ã¢â‚¬Å¾Ã†â€™ for 5 min, and slowly cooled down to room temperature. Human ÃŽ ±-thrombin (Tb), bovine serum albumin (BSA), and human serum albumin (HSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hemin, [tris(hydroxymethy-l)aminomethane] (Tris), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), 2,2-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), and H2O2 were purchased from Aladdin Reagents (Shanghai, China). A hemin stock solution (1 mM) was prepared in dimethyl sulfoxide (DMSO) and stored in the dark at −20  °C. All other chemicals were of analytical grade and were used without further purification. All solutions were prepa red using double-distilled water, which was obtained through a Milli-Q purification system (Billerica, MA, USA). Absorbance Measurements. Absorbance measurements were performed under room temperature using a TU-1901 UV−visible spectrophotometer (Beijing Purkinje General Instrument Co, Ltd., China). Kinetic data were recorded at the wavelength of 420 nm every 5 s during the first 5 min of the reaction. The absorption spectra of the solution was measured in the wavelength range from 390 to 490 nm. Procedure for Thrombin Assay. The experiments were performed in 50 ÃŽ ¼L of Tris-HCl buffer (20 mM Tris-HCl, pH = 7.4, 100 mM NaCl, 20 mM KCl, 1 mM MgCl2) containing 200 nM P1, 200 nM P2 and varying concentrations of Tb. The mixture was first incubated for 30 min at room temperature to allow complete binding. Next, 25 ÃŽ ¼L of 2 ÃŽ ¼M H1 and 25 ÃŽ ¼L of 2 ÃŽ ¼M H2 were added and incubated for 6 h at room temperature. Then, 20 ÃŽ ¼L of 2 ÃŽ ¼M hemin and 240 ÃŽ ¼L of HEPES buffer (25 mM HEPES, pH= 7.4, 200 mM NaCl, 20 mM KCl, 0.05% Triton X-100, 1% DMSO) were added, and allowed to incubate for 1 h at room temperature. Finally, 30 ÃŽ ¼L of ABTS and 10 ÃŽ ¼L of H2O2 were added to the mixture to give the final concentrations of 2 mM and 2 mM, respectively. The resulting samples were tested with a UV−vis spectrometer. RESULT AND DISCUSSION Design strategy for human ÃŽ ±-thrombin detection The sequences of the oligonucleotides used in this work were listed in Table 1. It consists of two proximity probes (P1 and P2) and two hairpin structures (H1 and H2). Both proximity probes P1 and P2 consist of four domains. Domain I includes two different thrombin aptamers, Apt29 (29 mer) and Apt15 (15 mer). The Apt29, orange domain of P1, binds to the heparin-binding site and the Apt15, skyblue domain of P2, binds to the fibrinogen-binding site of thrombin, resulting in proximity. Domain II (black) consists of a poly-T sequence that is designed to reduce the effect of steric hindrance induced by thrombin. Domain III (pink) is designed to have only 6 complementary bases, so that two proximity probes P1 and P2 cannot form a stable duplex without the target protein at room temperature. Domain IV (blue) is the key domain for binding-induced DNAzyme-assised signal amplification. By using two functional hairpin structures, the recognition of domain IV could trigger-on the hybridization c hain reaction that led to DNAzyme chains consists of the hemin/G-quadruplex HRP-mimicking DNAzyme. Hairpin structure H1 is functionalized at its 5 end with three-fourths of the G-quadruplex sequence, domain V (green), which is linked to the programmed sequences VIII (red) and VII (blue). One-fourth of the G-quadruplex sequence, domain VI (green), is extended at the 3 end of the hairpin H1. Hairpin structure H2 is functionalized at its 5 and 3 ends with one-fourth of the G-quadruplex (domain VI) and three-fourths of the G-quadruplex (domain V) sequence, respectively. Programmed sequences of domains VII and VIII in hairpin H2 are complementary to domains VII and VIII in hairpin H1, respectively. Both four domains in hairpin H1 and H2 are incorporated into a stable hairpin configuration in an initially locked format by hybridizing with their partially complementary sequences. It is noteworthy that sequence V is partially hybridized with domain VII in hairpin H1 or VIII in hairpin H2, w hich prevents the self-assembly of the active hemin/G-quadruplex DNAzyme. Principle of binding-induced DNAzyme-assisted amplification strategy for human ÃŽ ±-thrombin detection The working principle of human ÃŽ ±-thrombin detection is illustrated in Scheme 1. In the absence of thrombin, domain III in P1 and domain III in P2 will not associate since the complementary sequences (6 nt) are too short to promote efficient hybridization. When the target thrombin is introduced into the system, domain I in P1 and P2 bind to the protein simultaneously, resulting in domain III and III sufficiently close and to hybridize to each other to form a stable P1-Tb-P2 duplex, step 1. [BB2013-AM-3] Once the P1-Tb-P2 duplex forms, it associates with the stem region of H1, domain VII, leading to an opening of H1. This opening of H1 results in the release of the single-stranded domain VIII and the conserved three-fourths of the G-quadruplex (domain V), step 2. The released domain VIII then hybridizes with domain VIII of the stem in H2, and opens H2 using the strand displacement principle, step3. Subsequently, the liberated domain VII in H2 cross-hybridizes with H1 by hybridizatio n of domain VII to domain VII in H1, resulting in two G-quadruplex subunits (domain V and VI) sufficiently close and to self-assemble into a G-quadruplex structure, step 4. [AC2012-4] In the process of this autonomous cross-opening of H2 and H1, strand displacement can be repeated continuously, generating numerous G-quadruplex structures. In the presence of hemin, the resulting catalytic hemin/G-quadruplex peroxidase-mimicking DNAzymes catalyze the H2O2-mediated oxidation of the colorless ABTS2− to green-colored ABTS†¢Ã¢Ë†â€™. Detection of thrombin in human serum To further demonstrate the feasibility of the proposed method in real bioenviroments, we performed the detection of thrombin in human serum. [AC2013-4] Three concentrations of thrombin (10 pM, 100 pM, and 1000 pM) were spiked into 10-fold diluted human serum. [ZK-CC-1] Figure 4 shows the time-dependent absorbance changes of ABTS†¢Ã¢Ë†â€™ in response to different concentrations of thrombin. [AC2014-6] In logarithmic scales, the absorbance value exhibits a linear correlation with thrombin concentration over a range of 3 orders of magnitude from 10 pM to 1000 pM (inset of Figure 4B). [AC2012-12] The result indicated the potentiality of the proposed method for protein detection in real biological samples. [AC2013-4] Conclusions In conclusion, we have developed a binding-induced and label-free colorimetric method for protein detection based on binding-induced DNA hybridization and DNAzyme-assisted signal amplification. This method does not require any modification of DNA and involve any protein enzyme, which makes it technically label-free, enzyme-free and very cost-effective. Furthermore, the present approach uses a simple separation-free procedure in which the assay is conducted in a homogeneous solution.[AC2014-3] In addition, due to the excellent specificity of two proximity probes to the thrombin and the ingenious design of two hairpin structures,[AC-EA-2] this method exhibits a high sensitivity for thrombin detection, with a low detection limit of 2.5 pM. More importantly, this method can be extended to sensitive detection of other proteins by simple changing the aptamer sequences of the two proximity probes. To sum up, this simple and cost-effective colorimetric signal amplified method has great poten tial to be used as a universal tool for ultrasensitive analysis of thrombin or other proteins in serum and supply valuable information for biomedical research and clinical diagnosis.[ZK-CC-1]

Life in the 90s Essay -- essays research papers

Throughout the 1990’s there was a lot of good and bad times. Starting with the good things, I will begin my paper with 5 of the up-most important events in this period of time and then conclude with 4 of the down sides. Early in the 90’s was Desert Storm, which was a huge success. The 1991 bombing of Baghdad was a demonstration of smart, powerful weapons, planning ahead and military superiority. Having control in the air was a major goal of those who had planned out the attack. Starting attacks on the capital was the major idea and political goal of destabilizing Saddam Hussein's regime (basically the main purpose here was to have Iraq’s leader murdered). About 244 laser-guided bombs and 88 cruise missiles were delivered against Baghdad targets in 43 days of war. That’s most of the powerful weapons used in Desert Storm. Overall, around 250,000 individual bombs and missiles were dropped or fired in the 42 days. There were only 12 days and 12 nights when there were no strikes against Baghdad. There were only 14 nights when more than two targets were attacked within the city. For a lot of reasons, including growing political constraints on capital missions, fewer Baghdad targets were bombed as the war progressed. The campaign's end contrasted right after the opening night display. I think that Desert Storm could thank the war planners for the high achievements because they way they attacked Baghdad was almost like a sneak attack, and hey weren’t prepared, Overall, they were caught off guard. Next I will move onto wireless communication.   Ã‚  Ã‚  Ã‚  Ã‚  Now a new â€Å"fad† in the 90’s is things such as cellular telephones, everyone has them. Behind the calling process is more than you know before you purchase these items. When a cellular phone makes a call, it normally transmits it's a certain wave out to a cell phone site (towers). The number called in a short overwhelming piece of data. This data is the short buzz you hear after you hit the SEND button and before the tower catches the data. These things are the components the cellular provider uses to make sure that the phone is programmed so that it is billed and that it also has the identity of both the customer and the phone. Now kids starting at almost the age of 13 begin to carry these items. These are a high point of technology in the 90’s and everywhere you tur... .... Some schools districts that experimented with metal detectors for a short time found them a bit frustrating.And kids all over the world are getting guns from all these places but nobody is doing anything about it. It is very easy for someone to get a handgun. If we could stop the selling of drugs just by maybe 20% or even 15%, there would be a whole less violence due to the lack of money. So nobody would be able to buy a weapon. Teachers are in as much danger of being killed or attacked, as kids are at school. The recent examples are the series of shooting which took place in 1998 started with Columbine High School in colorado. The risk of a teacher being attacked by a student has doubled since 1956. This is a lot considering that you don't hear about too many teacher attacks on the news but you do hear about teens being killed. Approximately 100 teachers have been assaulted annually in the past four school years . Also last year in about six incidents which highlighted the newspapers about 25 students along with 5 teacher who were killed due to high school shootings. The big problem involving violence is how we can stop the violence from spreading and increasing.

Empathy in To Kill A Mockingbird Essay

In the grand scheme of things, each of us is working hard to see ourselves prosper. When we are fighting for survival, why should any of us take the time to feel for our fellow human beings? In her novel, To Kill a Mockingbird, Harper Lee implies that having the ability to feel for others or to show empathy not only benefits others, but can lead to personal gains as well. This is best demonstrated through the characters of Atticus, Jem, and Scout Finch. An obvious example of this claim is through the character of Atticus Finch. Because of Atticus’ ability to empathize with everyone, he is well respected by the town, even when he is doing some controversial things such as defending Tom Robinson. Atticus’ use of empathy is apparent during the trial, where Atticus is blaming Mayella Ewell for falsely accusing Tom Robinson of rape. Rather than explicitly attacking Mayella, Atticus says, â€Å"I have nothing but pity in my heart for the chief witness of state, but my pity d oes not extend so far as to her putting a man’s life at stake.† (Lee-203). Atticus is still delivering the same basic message, but rather than blatantly accusing Mayella of lying, he is empathizing with her and in a way justifying her actions, however wrong they may have been. By conveying his points in ways that do not make others feel like they are being personally attacked, Atticus is a well-respected member of society. We learn of the extent of this respect when Scout complains: â€Å"Despite Atticus’ shortcomings as a parent, people were content to reelect him to the state legislature without opposition. I came to the conclusion that people were just peculiar.† (Lee- 243). Even after he lost the controversial Tom Robinson case, the town still elected Atticus to serve on the state legislature since he was so respected because of his ability to empathize with each and every member of Maycomb. We also see this empathy in Jem, who clearly demonstrates more understanding by the end of the novel. We first get a glimpse of this after he helped end Ms. Dubose’s morphine addiction before her eventual death. After she dies, Jem receives a white camellia flower from Ms. Dubose. At first, Jem is angry, since he thinks Ms. Dubose is getting back at him, but Atticus explains how Ms. Dubose was a brave lady because she was able to end her morphine addiction  before she died. â€Å"Jem pic ked up the camellia, and when I went off to bed, I saw him fingering the wide petals.† (Lee-112). Jem is listening to Atticus’ advice and is trying to empathize with Ms. Dubose, whom he is finally able to respect. We see Jem’s newfound maturity develop throughout the novel. After Atticus loses the case, Jem begins to make sense of the world. â€Å"If everyone’s alike, why do they go out of their way to despise each other? Scout, I think I’m beginning to understand why Boo Radley’s stayed shut up in the house all this time†¦ it’s because he wants to stay inside.† (Lee-227). Jem is obviously maturing when he empathizes with Boo Radley, a character everyone despises despite not even knowing him. As he makes sense of the world, Jem begins to empathize with even the most unlikely of people, leading to increased wisdom. Even Scout, the least mature and most clueless of the Finches, learns the skill of empathy by the end of the novel. Initially, Scout was always quick to judge others and saw things only as black or white. She saw Aunt Alexandra as mean and unfair. However, she changes her opinion after seeing her aunt stay calm and ladylike even after ne ws of Tom Robinson’s death. â€Å"After all, if Aunty could be a lady at a time like this, so could I.† (Lee-237). Scout is beginning to respect Aunt Alexandra for her positive aspects, rather than showing disdain for her shortcomings. Scout also demonstrates a heightened sense of understanding to Boo Radley, specifically, when Boo wants Scout to walk him home. â€Å"I would lead him through our house, but I would never lead him home.† (Lee- 278). Scout understands that it would be embarrassing to Boo to have an eight year old girl leading him home and it would give possible onlookers the wrong impression. Instead, Scout had Boo hold her hand so it would look like he is walking Scout, which would seem normal. By empathizing with people she once did not respect, Scout has obviously come a long way from the immature little girl that she was at the start of the novel. Empathy is not just there to make us feel good about ourselves. Rather, the ability to empathize makes us better human beings and it lifts society up as well. The Finch family is a shining example of this ability to empathize, a s they combat racism in To Kill a Mockingbird.

Research Paper Chapter

Many companies introduce varieties of household cleaner and disinfectant that eliminate microorganisms effectively. Oxalic acid and Phenol acid are commonly found in household cleaner and disinfectant because they are the main ingredients in the elimination of the said microorganisms. Household cleaner and disinfectant nowadays are really expensive and contain many chemicals that might cause irritation to sensitive people.Eggplant (Solarium melange) is a species of nightshade commonly known in British English as barbering and also known Sebastian, bribing eggplant, melange, or Guiana squash. It bears a fruit of the same name that is widely used in cooking, most notably as an important ingredient in dishes such as mousse's and ratatouille. As a member of the genus Solarium, it is related to both the tomato and the potato. It was originally domesticated in India from the wild nightshade, the thorn or bitter apple, S. Incant. Oxalic acid and phenol acid occurs naturally in some plants i ncluding eggplant.Since eggplant contains Oxalic acid and Phenol acid which are the main ingredients in producing household disinfectant and cleaner the researchers name up with the idea on producing organic household cleaner and disinfectant out of eggplant. This product will help people that are sensitive in some products that contain high chemicals that irritate them. It will be cheaper than other commercially manufactured household cleaner and disinfectant that are sold in the market today. Background of the Study Nowadays, people have using different household disinfectant and cleaner that are expensive and less effective.This study aims to determine whether the Eggplant (Solarium melange) has the ability to be a good source of oxalic and phenol acid which are commonly used components for making household disinfectants and cleaners. Eggplant (Solarium melange) is rich in different vitamins that are useful in fighting diseases. Aside from being useful in fighting diseases, eggpl ants are also a rich sources of phenol acid that function as antioxidants which also contains a so-called chlorine acid. This is responsible for the eggplants' bitter taste when their flesh is cut.This is an enzyme called polyphony oxides that triggers a phenol acid that produces brown pigments. Eggplant s among a small number of foods that contain measurable amounts of oxalate's, a naturally occurring substances found in plants, animals, and human beings. When oxalate's become too concentrated in body fluids, they can crystallize and cause health problems. But this oxalate's is can also be used as cleaning purposes having a bleach-like quality that is perfect for making household disinfectant or control bacteria and germs.Overall, using eggplant in making household disinfectants and cleaners has benefits since it contain properties that can be used as an agent for cleaning. Research Problem: The study aims to determine the anti-bacterial properties of Oxalic acid and Phenol acid of eggplant (Solarium melange) . Sub- Problem: What is the zone of inhibition of the Blended and Boiled eggplant extract? Which is more feasible to use as a disinfectant? Blended or Boiled eggplant extract? Is the eggplant extract feasible to use as a Household Cleaner? Why?Hypothesis: Alternative: The oxalic acid and phenol acid of eggplants are effective disinfectants and cleaning agents. Null: The oxalic acid and phenol acid of eggplants are not effective disinfectants and cleaning agents. Significance of the Study This study wants to determine the feasibility of eggplant (Solarium melange) as cleaner and disinfectant. The results that would be gathered would be beneficiary to both the consumers and producers of products. The producers of products won't have to look for an expensive material, thus saving them money in production.The consumers would be able to have a product in a lower price and something produced out of a material they normally use only for food consumption. The ra rity of such product could also boost its standing in the market nowadays. There would also be little to no harm to the environment and to anyone who will use the product. Eggplant (Solarium melange) also known as barbering in British English is a species of nightshade, it is contains vitamins, minerals and Photosynthesis which aids in antioxidant activity. It contains 0. 9 g/grammas of Oxalic acid, an odorless, white crystalline solid that exists in its natural state in many plants ,a component of metal cleaning agents and a scouring agent in textile cleaning, striping and finishing, if inhaled or absorbed through skin can caused kidney damage among other things. Eggplants also contain phenol acid Plants form these compounds to protect against oxidative stress, as well as infection by bacteria and fungi. One of the active ingredients of some household disinfectant, is Phenols.Both of these compounds are contained by Eggplant (Solarium melange), which makes it an even better candida te for finding out it's possible usage as cleaner and disinfectant. It is cheap, abundant and environment-friendly. It could also be potentially good in terms of being in commercial market because of its unique ensues. Scope and Delimitation Scope The coverage of this study is to know about the effectiveness of an eggplant (Solarium melange) as cleaner and disinfectant. The objective of this study is to produce a cheaper disinfectant that is effective for household cleaning.The product is used to clean tight and dirty places and users of the product must be 7 years of age or above. Materials used for the disinfectant are body of the eggplant, water, Sodium Beneath (for durability), etc. That are mixed together by a blender. The study and the experimentation will be finished within a week. The purpose of the product is for cleaning and disinfecting. Delimitation The study does not cover the comparison of the effectiveness of the eggplant from other plants that can also be used as cle aner and disinfectant (such as calamines, etc. ).The study is not allowed to be conducted by any person below 1 0 years of age. The study does not include other dirty objects but only limited to tight and dirty places such as bathroom, etc. Definition of terms Conceptual Definition Blender – It is a kitchen and laboratory appliance used to mix,puree, or emulsify food and other substances. Boiling Method – It is the method of rapid vaporization of a liquid, which occurs when a liquid is heated to its boiling point, the temperature at which the vapor pressure of the liquid is equal to the pressure exerted on the liquid by the surrounding environmental pressure.Chemicals – It is a form Of matter that has constant chemical composition and characteristic properties. Alt cannot be separated into components by physical separation methods. Cleaner – It is a type of industrial or domestic worker who cleans homes or commercial premises for payment. Unlike other ser vants, their primary task is cleaning. Disinfectant – It is a substance that is applied to non-living objects to destroy microorganisms that are living on the objects. Eggplant – It is a species of nightshade commonly known in British English as barbering.It bears a fruit of the same name that is widely used in cooking, most notably as an important ingredient in dishes such as mousse's and ratatouille. Extraction – It is the process of obtaining something from a mixture or compound by physical or chemical means. Microorganisms – It is an organism that is microscopic. It includes bacteria, fungi, and prisons. Night shade – It is n economically important family of flowering plants. The family ranges from herbs to trees, and includes a number of important agricultural crops, medicinal plants, spices, weeds, and ornamental.Many members of the family contain potent alkaloids, and some are highly toxic, but many cultures eat nightshades, in some cases as a staple food. Oxalic – It is an organic compound with the formula HECTIC. It is a colorless crystalline solid that dissolves in water to give colorless solutions. It is classified as a diacritically acid. In terms of acid strength, it is much stronger than acetic acid. Oxalic acid is a reducing and its conjugate base, known as oxalate (CACHE-?), is a chelating agent for metal actions.Phenol- It is any analogous hydroxyl derivative of benzene. Shelf life – the length of time that a commodity may be stored without becoming unfit for use Sodium Beneath – It has the chemical formula Nachos; it is a widely used food preservative, with E number EYE 1 . It is the sodium salt of benzene acid and exists in this form when dissolved in water. It can be produced by reacting sodium hydroxide hedonistic acid. Benzene acid occurs naturally at low levels n cranberries, prunes, greengage plums, cinnamon, ripe cloves, and apples.Operational Definition Blender- It is an applianc e that will be used to mix the eggplant with water. Boiling Method- It is the method that will be used to extract the content of the eggplant by means of boiling it in ICC – ICC temperature. Chemicals- It is the substance that would be extracted from the eggplant. Cleaner- It is the product that would be made from the eggplant's extract. Disinfectant- It is the product that would be made from the eggplant's extract. Eggplant – It is the vegetable that would be used to make a household cleaner and disinfectant.

Animal Management assignment Essays

Animal Management assignment Essays Animal Management assignment Essay Animal Management assignment Essay Risk assessment for chicken transportation from a farm to a slaughter house (2 of 2) 12. References 13. References Free range chicken management system Introduction Poultry is one of the most popular meat products in the I-J. EX. marketing rules require that free-range poultry farming allows chickens to run free giving them a chance to spend at least half of their day in the open air. European marketing standards require that free-range chickens have access to an open-air run in the day time in the night time chickens are let inside to protect them from predators. The birds should have the option to go inside when weather conditions are bad. In a free-range management system a chickens life span is longer than caged chickens, it also causes less stress to chickens in comparison to any other management system. This system requires minimal Costs, but moralities due to predators are quite high (Amelia J. G. 1999). Free-range chickens have an additional opportunity to show their natural behavior. It gives them more space for exercising and they are able to get natural sunlight (DEFRAY 2001). Access outdoors reduces the level of ridding for birds that choose to stay inside. Also more space and enrichment prevents chickens from cannibalism and feather pecking. Housing EX. marketing laws require that free-range chickens have access to open-air runs for at least half of their lives. There must be at least alma of range per bird. (Compassion in World Farming March 2013) Substrate in the shelter needs to be straw like or wood shavings; this is so chickens can display their natural behavior such as digging, dust bathing, pecking etc. Outside substrate has to be free draining and covered mainly in vegetation (DEFRAY 001 ), (Fig. 1). The area has to have some enrichment for birds like trees, bushes, hay piles etc. The roost must provide shade from the sun shelter from the wind and aerial predators, real or apparent (planes, other vehicles, humans etc. ). The building has to have plenty of open access for chickens to go in and out as desired. The housing needs to have easy access in all weather conditions for vehicles to park for bird transfers. Fig 1. Free-range chicken farm (Mutton p. 2006) Ventilation The shelter must have natural automatic ventilation control (side vents along he length of the housing, above the windows). The ventilation system needs to function so the air change rate is adequate to remove the heat from the building during hot weather. This is referred to as the maximum ventilation rate. During periods of low ventilation cold weather it needs to be able to remove stale/unpleasant air and humidity (minimum ventilation rate). Confirm and draught free distribution of air within the building is important. (DEFRAY Publications, 2001) Ventilation can be produced by natural forces or by powered fans (Fig. 2), either way the birds need to have the right enraptures at all times. Good ventilation is essential, especially in the summer time when weather gets hot. If ventilation is poor the birds might overheat, this could lead to stress and in the worst case, death. Ventilation helps to lower ammonia levels in closed areas; it also provides fresh air and reduces unpleasant doors. Fig. 2 Building ventilation system (SHEIKH, F. 2012) Layout feeders perches other equipment It is essential for feeders, drinkers, nest boxes and perches to have the right layout, Otherwise it could cause stress lower production. Feeders and drinkers (as shown in a Fig. ) are installed along the entire roost; this gives a chance for every chicken to have access to food and water. This layout reduces chances of starvation and dehydration. Perches should be arranger in such a way that chickens can freely move between them and other equipment, if not the birds could get hurt. Moreover, if the birds were to get damaged it could compromise the resale of the product. All equipment should be mobile and easy to move/remove for cleaning or when gathering the birds for transportation. Lighting Lighting needs to be designed and installed evenly throughout the building etc reduce an equal amount of artificial and natural light. For health and safety reasons the lighting system must be raised high enough so that the birds cannot reach peck it. Conclusion Free-range farming is beneficial for birds and consumers. For chickens it means that they will have slightly longer, healthier and more fulfilled life. Moreover, it provides higher quality welfare along with opportunities to go outside and roam, whilst offering a more enriched life inside. Slower growing bird breeds with access to open-air produces less but better fat and a higher quality product (RASPS, 201 1). Free range farming is beneficial to the consumer as the birds receive higher welfare, tend to be higher quality product, reasonably priced and are kept in an environment that is more natural. There are many issues surrounding the sustainability of agriculture, including restoration of habitats, improving soil health and water quality. The unsustainable practices that are uneaten have an ill effect on the aforementioned and the environment as a whole. The world population is growing at a fast rate which leads to the demand of agricultural commodities. Moreover, this leads for a call to action in sustainable agriculture management to support demands, an area of concern is water control. Water is the life blood of agriculture and wildlife alike, poor water management can have an impact on crop grow/regeneration to habitat disruption. An example of poor water management affecting crops is in the Irish grasslands, the grasslands have poorly drained soil which impedes the growth and yield of crops. To resolve the issues surrounding this area it has been suggested to improve the natural flow of the water off of the land by creating properly managed hedgerows, wetlands and buffer zones. Therefore, creating a lance that can see the sustainability of wildlife and crops equally, rather than only focusing on the yield of crops alone. Another area that is causing concern in agricultural water management sustainability is the impact it is having on the habitat of wildlife in general. Agriculture has a deep impact on the water resources within watersheds. When wildlife habitats are converted into agricultural land the effects alter the diversion of water, through the use of pesticides and removal of plants/water etc. To sustain these habitats plant diversity and water sources in the agricultural surrounding areas should be maintained, this will support a diversity of wildlife. The sustainability of wildlife and agriculture go hand in hand, if a natural ecosystem is upheld in the surrounding areas it could provide a natural source of pest management. In a world that the population could reach 9 billion by 2050, demand must equal supply. The requirement for agricultural sustainability is fundamental for the future of the environment and human demand. An ongoing strategy must be implemented and upheld to conquer the issues that are present, this will help ensure demand is met but not adversely affecting wildlife or the environment in the process. Agricultures deep connections to the world economy, human societies and biodiversity make it one of the most important frontiers for conservation around the globe. (World Wildlife Fund 2015) Zoo management nationally and internationally The diagram above is a tree structure of coordination in zoo management. The following will describe each organization and outline their activities: CNN International Union for the Conservation of Nature or World Union for the Conservation Of Nature. The JINN is responsible for valuing and conserving nature, it also provides information to attempt to solve major sues in climate change, food and overall development. GAZA The European Association of Zoos and Aquaria is a community that ensures that its zoo members throughout Europe uphold the highest standard of care. This relates to breeding and protecting endangered species. PEP The European Endangered species Programmer has workers collecting vital information on endangered species. They present a plan to ensure the species are protected and are managed correctly in the future. ESP. The European StudBook keeps data on all births, deaths and transfers of animals in Europe. This data can be used to advise zoos on how to manage healthier environment if required. TAGS Taxonomic Action Groups advises on how captive rearing should be managed and what space should be allocated for each species, alongside conservation. PIZZA British and Irish Association of Zoos and Aquaria is an organization that represents the zoo and aquaria community in Britain and Ireland, it provides a voice for its members. WAS -? World Association for Zoos and Aquaria encourages relationships between zoological gardens and aquariums with the intent of promoting the highest level animal welfare, it also represents both communities. ISIS International Species Information System is a medium for international sharing of knowledge of animal species in animal management and conservation. Sims Zoological Information Management Systems goal is to improve local care by promoting population health and international conservation. SPARKS Single Population Animal Records Keeping System analyzes, records and manages the animal data, usually its focus is a single species. ARKS Animal Record Keeping System collects key data on animals in zoos to provide a management inventory. Medusas Medical Animal Record Keeping System works with ARKS to link he animal inventory to medical records. This data can be used to provide medical care advice and when tests are required. Animal identification methods Identification method Animals the method can be used on Application Use Implications for the welfare of the animals Ringing/ banding Wild domesticated birds. Small, individually numbered/marked metal or plastic tags are put on to the leg or wing of the bird. To identify individual birds, to track bird migration population. Causes stress in the process of catching and ringing/banding. Ear tagging Domestic livestock: cattle, sheep, pigs goats. Two plastic or metal tags that have identical numbers are joined through animals ear. To identify individual animals, to record and trace their life since the moment they are born to their death. The procedure might cause stress and pain. Freeze branding Horses cattle. The iron is cooled to a temperature of between -160 to -ICC. The branding iron is then pressed onto a shaved patch of skin on the animal. Is manly used by farmers to identify animal ownership. Causes pain and stress. Marking is permanent. Ear punching Mainly rodents. Could be used on cats and dogs. Using a special punch to produce a small notch in the animals ear. To identify rodents. Mainly used in laboratories. Causes pain, stress and is permanent. The Microchip Cats, dogs rabbits. The Microchip is implanted under an animals skin. To identify lost pets or other animals. Can cause stress, pain and is Permanent. Chip might move under the skin. Identification collars Companion animals. The collar is placed around the neck with important contact information. To identify individual animals, helps to find the owners of lost animals. Can be easily lost. Can cause damage to the animal or suffocation. Acoustic tag Fish.