Galatians Bible Argument Essay - 2123 Words

I. Introduction 1:1-9 In this opening chapter, Paul begins with a formal introduction of himself. He asserts the source from whom he obtained his apostolic call and ministry from- the risen Christ and â€Å"God the Father† (v.1). He follows with a stern rebuke of the Galatian Christians who have allowed themselves to be duped into to embracing the heretical teachings promulgated by the Judaizers. A. Salutation 1:1-5 Paul establishes his credentials forthrightly and formally. This was crucial in terms of validating the ensuing message of the epistle. His readership, as well as his critics (the Judaizers), needed to be reminded of his apostolic authority as he attacked the heresy that had taken foothold in the Galatian Christian community.†¦show more content†¦A. Paul’s Call 1:11-12 Paul explicates the particulars of his divine commissioning. Again, this was necessary so at to â€Å"re-authenticate† his apostleship before the Galatian believers (and his dissenters). B. Paul’s Confirmation 1:13-2:21 1. His Early Activities 1:13-17 Paul discusses his conversion from Judaism. This is significant as he prepares to dismantle the notion that one can be justified by observing the Law. His unique claim is that he was so zealous for Judaism that he actually persecuted the church. This devotion distinguished him from his countrymen, and reputably so. It was also â€Å"credential† that qualified him to challenge the heresy of the Judaizers. 2. His First post-Christian Jerusalem Visit 1:18-24 Paul goes up to Jerusalem and meets Peter and James. Presumably, they, in addition to other Christians in the churches in Judea acknowledged Paul’s apostleship as being authentic (v.24). Paul is gradually mounting a convincing case against the Judaizers; they criticized him wanting to â€Å"enhance his reputation† by preaching his gospel13 3. His Second post-Christian Jerusalem Visit 2:1-10 Paul re-visits Jerusalem 14 years subsequent to his initial visit to the city to meet with the apostles. In this section, he states that they saw that he â€Å"had been entrusted with the gospel to the uncircumcised†. The recognition by the original apostles (i.e. Peter, James, and John) regardingShow MoreRelatedSouthwestern Assemblies Of God University. . . Hermeneutics1734 Words   |  7 PagesWhat’s the Why? The book of Galatians was written for the simple reason to clear up the truth of the gospel. Shortly after Paul left the church of Galatia, an unknown person or ‘people’ came to the church and led them to believe otherwise. The church of Galatia, being brand new was thrown off by the idea that they were living an immoral life, and immediately changed what they were doing to fit what the people or persons taught this. The thought of having the Galatians taken from the truth infuriatedRead MoreDate and Destination of Galations2324 Words   |  10 PagesGrace School of Theology NORTH OR SOUTH: Destination and Date of Galatians A Paper Submitted to Gregory P. Sapaugh, Th.M. Ph.D In Partial Fulfillment of the Requirements of RS-503: Research Methods, Writing, and Technology by William (Brant) Wallace Houston, Texas February 25, 2015 Contents Introduction 1 General Debate 1 Geography of Galatia 2 Diversity and Strategy 2 Advantage for Missional Activities 3 The North 3 Evidence for the NorthernRead MoreMaddie Piner Final Bible Paper: Galatians Paul begins his letter by saying he is an apostle not600 Words   |  3 Pages Maddie Piner Final Bible Paper: Galatians Paul begins his letter by saying he is an apostle not from men nor through man, but Jesus Christ. He sends grace and peace from God and Christ, stating the deliverance made possible by Jesus death for our sins. As a servant of Christ, Paul is seeking to please God not man. The gospel preached by Paul was being twisted by those false teachers. Paul proceeds to defend both the gospel and his apostleship by showing thatRead MoreReasons For Not Accepting Acts As A Historical Record1534 Words   |  7 Pageshowever, would argue that though Paul was once a Jewish leader, he experienced a conversion and helped start the new Jesus movement. He then preached what he believed about Jesus to anyone, despite much controversy. In order to dive into these arguments, it is important to address the validity of sources used. One source I will be using is the book of Acts. According to Richard Pervo in The Making of Paul, there are many reasons for not accepting Acts as a historical record. He suggests reasonsRead MoreExplication of Sonnet 144 Essay859 Words   |  4 Pagesregards to who it is written about and the story behind it. I would like to state first of all that the straight facts about the sonnets are so few and that theories and debates are many. Doubt is cast over nearly every aspect of these sonnets. Arguments from when they were written, whom they were written to, why they were written, and even in many cases the question of who wrote them. The common thought of whom they were written to and why they were written has evolved as of late to reach a viewRead MoreAnalysis Of The Article The Pericope Of Galatians 2 : 15-211187 Words   |  5 PagesThe pericope of Galatians 2:15-21 is the thesis o f the entire letter to the early Christian communities in Galatia (Furnish 1971). It begins after Paul greets the people of Galatia. The people of Galatia have been visited by missionaries who still insist that the laws must still be adhered to especially the laws of circumcision causing confusion in the people. Peter had drawn away from the gentiles over purity issues and this was still greatly on Paul’s mind in writing to the Galatians (Furnish 1971)Read MoreThe Bible ( Chr 105 Oa2 )1558 Words   |  7 PagesTabitha Sanders Introduction to the Bible (CHR 105-OA2) Anderson University December 5th, 2016 Final Exam 1. Using the book, notes, and biblical text itself, choose two of the Gospels and compare and contrast their presentation of the life of Christ. Be sure to include how things like the authors’ background and audience affect their message. I have decided to compare and contrast the Gospels of Matthew and Mark. In the book of Matthew, we begin with the angel coming to Joseph and telling him ofRead MoreWomen in Ministry and the Redemptive Trend Hermeneutic: My View1617 Words   |  7 Pagesout one organization for focused argument, but this statement accurately represents a sentiment within the faith that I find disturbing. In this paper, I will use the redemptive trend hermeneutic to deconstruct the CBMWs affirmation, while providing my own views on why I find both women in ministry and the redemptive trend hermeneutic as valid. The redemptive trend hermeneutic is a strategy for making sense of our 2000+ year old collection of texts we call the Bible. Keeping in mind the fallennessRead MoreJustification by Faith Essay1162 Words   |  5 Pagesalone cannot save them. Verse 16 Knowing that a man is not justified by the works of the law, but by the faith of Jesus Christ. this is one of the clearest definitions in Scripture regarding the way in which we can become justified. Here in Galatians 2:16, justification deals with the fact that we cannot be justified#8212;or given good standing before God#8212;through our obedience to the Law of Moses. According to Paul, it must be given to us by grace alone, through faith alone, in ChristRead MoreGalatians 3 : 15-29 The Law And The Promise3072 Words   |  13 PagesGalatians 3:15-29 the Law and the Promise General Introduction Galatians author was the apostle Paul and the audience was the churches of Galatia, discovered by Paul in his first route of mission work or spreading the gospel. The Galatian Epistle was addressed to the â€Å"Christian congregation of Galatia† (1:2). Certain positons are held by certain scholars as to who Paul’s letter to the churches of Galatia was written. One theory is that it was written in Northern Galatians. A view held by J.B. Lightfoot

Comparison of The Matrix and Do Androids Dream of...

Comparison of The Matrix and Do Androids Dream of Electric Sheep? Andy and Larry Wachowskis movie, The Matrix parallels many of the characters and themes that are explored in Philip K. Dicks novel, Do Androids Dream of Electric Sheep?. There are five main characters or groups of people that are explored in Dicks novel. First, Morpheus, the leader of the unplugged individuals, emulates the role that the Rosen Association plays. Next, Cypher, an underhanded man, who is comparable to Rachel Rosen, a typical android, shows no empathy towards anyone. Surprisingly, Rick Deckard, an android killer and the main character, resembles the agents in The Matrix. Following Rick, the androids represent the enlightened men and women in The†¦show more content†¦It may be too much for their minds to handle. In addition, both Morpheus and the Rosen Association are constantly searching for something more. Morpheus is in search of the one, a man that is vastly superior to all others. Likewise, the Rosen Association is constantly looking for new ways to make androids more intelligent and more human- like. Ricks boss is telling him about the android that killed a senior bounty hunter, It must have been one of those new, extra-cl ever andys the Rosen Association is turning out(28). Morpheus wants to find the one who will be able to challenge the agents who police the matrix. The Rosen Association wants to create an android that is so realistic, that it is impossible to tell it apart from a human. The problem is, these creators are unable to form an android with the emotion of empathy. More specifically, Rachel Rosen, an android lacking empathy, is surprisingly consistent with Cypher, a deceitful, unplugged individual. Neither of these characters seem to have any empathy at all. In Rachels case, it is expected because she is an android. She insists on helping Rick find and retire other androids. She offers her services to Rick Deckard without wanting anything in return. Rick is reluctant, but she is persistent, But I really think you need me(90). She has absolutely no sense of feeling for othersShow MoreRelatedProject Mgmt296381 Words   |  1186 Pages4] 10.3.3.1 Lessons learned [8.3.3.4] 9.4.2.2 Individual performance appraisals Chapter 7 Managing Risk Chapter 15 Chapter 16 International Projects Oversight 11.1 Risk management process [F.8] 11.2 Identifying risks 11.3.2.2 Impact matrix 11.4 Risk assessment 11.5 Risk responses (.2–.1.2) 11.6 Risk register 7.1.2.5 PERT analysis 7.1.2.6.3 Contingency reserves 7.3.3.4 Change control management G.7 Culture awareness 1.4.4 Project offices 8.1.2 Continuous improvement 5.1 RequirementsRead MoreDeveloping Management Skills404131 Words   |  1617 PagesSUPPLEMENTARY MATERIAL 24 Diagnostic Survey and Exercises 24 Personal Assessment of Management Skills (PAMS) 24 What Does It Take to Be an Effective Manager? 28 SSS Software In-Basket Exercise 30 SCORING KEY AND COMPARISON DATA 42 Personal Assessment of Management Skills 42 Scoring Key 42 Comparison Data 42 What Does It Take to Be an Effective Manager? 43 SSS Software In-Basket Exercise 43 PART I 1 PERSONAL SKILLS 44 45 DEVELOPING SELF-AWARENESS SKILL ASSESSMENT 46 Diagnostic Surveys for

Worker Reduction Free Essays

FastServe has a tough decision to make. The five employees on the list have their own sets of good and bad points. Termination of an employment needs to be handled with care to avoid facing three labor suits. We will write a custom essay sample on Worker Reduction or any similar topic only for you Order Now Being a direct marketing organization, what the company needs are people who have the skills that are useful in its kind of business. Since the company is closing down its online marketing and distribution channels, it does not need people whose skills are limited on those areas. It needs to retain people who can make positive contributions to its main business. The first consideration that FastServe must take note of is how that employee will contribute to the direct marketing business. In line with this, FastServe takes productivity into serious consideration. Next, the company must also take into account other things like compliance with the company’s policy on attendance, work performance, productivity, and work redundancy. Another important thing to consider is the employees’ work status, whether they are full-time, contractual, or under a labor bargaining agreement. This is a very important consideration to avoid abuse of the Employment At Will doctrine. Finally, the company must take into account the different anti-discrimination in the workplace laws . Each person on the list could file for discrimination when terminated because each one of them falls under the protected minority. Before FastServe will have to choose the final three people for termination, the company must talk to each one of them and give them time to defend themselves or improve on weak areas. Even if the layoff is not on a large scale basis and would not violate the WARN Act, or the Worker Adjustment and Retraining Notification Act, it still would be humane and preventing legal actions if the company would do this act. Carl Haimes – Retain Of all the employees on the list, Carl Haimes has the best record. His overall track record performance is above average, the highest and only one in the group. In terms of productivity, he also scored above the median, which is what the company would like to see among its employees. In terms of education, Haimes has a degree in Information Systems, a skill that is useful in marketing. He is also a qualified systems analyst, an important asset to a marketing company that needs to have marketing systems in place. Haimes’ computer maintenance skills and LAN installation skills for three platforms will come handy for the maintenance of the company’s databases and electronic marketing portfolio. Haimes will also be useful when the company will be automating its systems. Strictly from skills and qualifications, Haimes is a clear candidate for retention. To improve Haimes’ chances for retention, no one has made any complaint on his behavior or work ethics. Moreover, the company has no problem with his attendance, the only one in the group. Haimes’ contract need not be an issue since the company would be retaining him. Haimes is gay and his sexual orientation could become a problem in conservative and traditional companies. Fortunately, FastServe, being a marketer, could not afford to be intolerant to diversity in the workplace. Diversity is what it needs to survive in a highly competitive environment. Retaining Haimes would benefit the company because of his skills, qualifications and adherence to company policies. Lastly, Haimes can’t be terminated because there is nothing that the company would find undesirable in his performance. Besides, Haimes could file for sexual discrimination if he gets terminated knowing that among the five, he is the most qualified and the best performer. Brian Carter – Terminate In Brian Carter’s case, the company must be very careful that it won’t be sued with violation against the Disability Act of 1995 since this employee has a carpal tunnel syndrome. Should Brian passed the medical tests, then, FastServe cannot terminate him because of this problem. Brian cannot continue with his employment with FastServe for various reasons even if he does not suffer from a wrist problem. First, Brian’s skills are highly specialized and limited to programming, which is very useful in the online distribution channels but not on direct marketing. Brian, being a techie guy, is unlikely to fit in a direct marketing kind of work even if he had a chance to train for marketing-related functions. He is a computer kind of guy and would feel more comfortable facing a workstation instead of facing people. Besides, Brian won’t find it difficult to find new employment since his skills are highly sought after in todays environment. The company can terminate him at will because his expertise is not needed anymore. Second, Brian has a staggering 17 days absences in a two-month period, the highest among the five of them, and a fact that made his supervisor dislike him. He could have improved on his performance and productivity if Brian weren’t absent for that many days. Terminating Brian would benefit the company because he will become an underperforming asset should his employment with the company continue. Sarah Boyd – Retain Sarah Boyd is the second employee that FastServe should retain. She’s with the company for 15 years and her status is full time. But her retention are not simply based on those two factors. Of the three remaining persons on the list, Sarah has the lowest number of days absent in a two-month period. Her overall track performance is average while her productivity is median. Sarah is capable of managing and administering an office, skills that are useful in a marketing company. Also, she’s the only person who knows office procedure better than anyone else. Her prioritizing and sorting skills could become redundant when the company will automate its processes. However, it would take time for the automation to be implemented. While waiting for that time, Sarah could continue doing her job and she could be taught other skills. Besides, Sarah is 53 years old and protected under the Age Discrimination in Employment Act of 1967. So long as Sarah is able and capable of doing her tasks, the company can’t make her retire against her will. However, should the company find the need to replace Sarah when she reaches 55 years old, FastServe could offer her an early retirement package that is attractive, fair and equitable. Nora Manson – Terminate Nora Manson’s overall performance is above average but her productivity is below median. The company is currently focused on high productivity from their employees and Nora falls short of that goal. There is also the question of Nora’s absenteeism that has already accumulated into two months. This is very high and would raise questions on her job loyalty. Another point against Nora is on her loyalty to FastServe. She has been directing employees to file discrimination complaints against the company she works for. Nora’s participation in discrimination charges against FastServe is protected by Title VII of the Civil Rights Act of 1964. These acts may be in accordance to her personal values and are protected by law but these do not show loyalty to FastServe. Being an advocate of fair treatment need not mean going to the labor court right away. Nora could have acted as intermediary to prevent labor suits. On the positive side, Nora has rescued two big accounts and is skilled in handling irate and upset callers. She’s also skilled in other aspects of making sales. Despite her positive contributions, Nora is too radical to become an asset to the company. While the company cannot take it against her to be watchful of discrimination, her work shouldn’t have been affected by all of her non-work related activities. Her activities with her affiliation could have been the reason why Nora’s productivity is low. FastServe should end Nora’s employment so long as her contract allows the company to do that. If the employment is for a definite period of time, then the company can use her performance, productivity and absenteeism problems as the ground for her termination. However, the company should be prepared to handle discrimination complaints that Nora may file against it considering that she is an African-American and is an active member of NAACP. Jenny Mills – Terminate Jenny Mills should also be terminated. Although the law prohibits termination of women because of pregnancy, the company won’t be ending Jenny’s employment because of that. She is on contract and her attendance is very bad. She is also taking extra breaks that are not supposedly allowed and this make male employees complain. Jenny cannot use as an excuse her pregnancy for the 14 days of absence in a period of one month. Pregnancies are mostly difficult in the first two months and once a woman is already beyond that, her condition would be better. Jenny is already five months pregnant and there are no indications that it is a difficult pregnancy. Although she holds a degree in public relations, she has no special achievements. Her skills are also limited to answering queries from customers and troubleshooting common instruments problems. These skills can easily be taught to other persons. The company can even delegate these tasks to Sarah Boyd so that when it is time to automate, Sarah would be useful in other areas. How to cite Worker Reduction, Papers

Paddy Clarke (1181 words) Essay Example For Students

Paddy Clarke (1181 words) Essay Paddy ClarkeThe novel Paddy Clarke Ha Ha Ha has no authorial presence at all, yet the readergains a richer understanding of the situation than Paddy ? or any other10-year old ? could ever have. With regard to the parents break up, howdoes Doyle achieve this? There are many factors which suggest how Doyle hassucceeded in creating a triangular relationship between himself the reader andthe narrator ? Paddy Clarke ? so that the reader has a greater awareness ofthe predicament that Paddy is in. Doyles achievement is how he alternates thepoetic and realistic without once lapsing into stream-of-self-consciousness; theonly way we as readers can tell its written by an adult, is by the spelling. We see the violence in Paddys life peripherally; Doyle tells us nothing morethan what the child sees and comprehends. One of the reasons for Roddy Doylessuccess lies in creating a realistic and convincing character for a 10-year oldchild. He does this by his clever use of language, and also in how he arrangeshis sentences to convey deep emotion and feeling than any emotive languagecould: Hed hit her. Across the face; smack. I tried to imagine it. Itdidnt make sense. Id heard it; hed hit her. Shed come out of thekitchen, straight up to their bedroom. Across the face. ? P190 In thisinstance, Doyle has used short and evident sentences, to invoke a feeling of aweand confusion. The short sentences represent how Paddy is dumbstruck and lostfor words, shocked by what hes heard ? this is also highlighted when hesays here; I tried to imagine it. It didnt make sense. Here, he alsoemphatically uses onomatopoeia ? smack, ? which adds to the sense offearful respect and also Paddys child-like inte rpretation of events. Repetition is used here ? Across the face ? heading his oft-repeatedamazement. Another example of how Doyle uses repetition can be seen on pages 153and 154: I waited for them to say something different, wanting it Only now, all I could do was listen and wish. I didnt pray; there wereno prayers for this. But I rocked the same way as I did when I was sayingprayers.I rocked Stop stop stop stop ? . Doyle uses repetition to showPaddys anxiety, when he repeats ?stop. Here, Paddy is mentallycommanding his parents to stop in desperation, as he thought he had done on page42: Stop. There was a gap. It had worked; Id forced them to stop. Hebelieves that he has the power to make his parents stop arguing, as shown onpage 42, but realisation dawns when he repeatedly tells them to stop on page154, and it doesnt work. This reflects on the fact that Paddy Clarke is achild, and his inability to restrain his emotions is a facet of his youthshowing through. Another childish aspect throughout the book is how Paddy ?like other children at that age would ? spouts offhand irrelevant knowledgethats hes picked up from class or elsewhere: Snails and slugs weregastropods; they had stomach feet. The real name for soccer was associationfootball. Association football was played with a round ball on a rectangularpitch by two sides of eleven people Geronimo was the last of the renegadeApaches I learned this by heart. I liked it. Readers can relate tothis, as we can all remember when wed learnt something that wed foundparticularly fascinating at school or the library, and recited it all the time,thinking we were clever. Another reason why the reader of Paddy Clarke Ha Ha Hahas a higher understanding than is simply because the adult audience has moreexperience in family issues ? from our own experiences. We can see theviolence in his life superficially; we are told nothing more than what the childsees and comprehends. A good example of this can be found on page 95: Ma saidsomething to Da. I d idnt hear it. I looked at ma again. She was stilllooking at Da. Catherine had one of Mas fingers in her mouth and she wasbiting real hard ? she had a few teeth ? but Ma didnt do anything aboutit. Here, Paddy has given us an insight to the emotional turmoil that existsin the family, but Doyle ? again ? has not used any emotional adjectives toshow this. We can interpret what is happening from his parents actions, whichjustifiably speak louder than words. Paddys mother is staring at Da, waitingfrom him to answer, and the baby is biting into her finger, hard as Paddy says. .u3e839ee77504ea9fd072faa7f5c83eb3 , .u3e839ee77504ea9fd072faa7f5c83eb3 .postImageUrl , .u3e839ee77504ea9fd072faa7f5c83eb3 .centered-text-area { min-height: 80px; position: relative; } .u3e839ee77504ea9fd072faa7f5c83eb3 , .u3e839ee77504ea9fd072faa7f5c83eb3:hover , .u3e839ee77504ea9fd072faa7f5c83eb3:visited , .u3e839ee77504ea9fd072faa7f5c83eb3:active { border:0!important; } .u3e839ee77504ea9fd072faa7f5c83eb3 .clearfix:after { content: ""; display: table; clear: both; } .u3e839ee77504ea9fd072faa7f5c83eb3 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u3e839ee77504ea9fd072faa7f5c83eb3:active , .u3e839ee77504ea9fd072faa7f5c83eb3:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u3e839ee77504ea9fd072faa7f5c83eb3 .centered-text-area { width: 100%; position: relative ; } .u3e839ee77504ea9fd072faa7f5c83eb3 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u3e839ee77504ea9fd072faa7f5c83eb3 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u3e839ee77504ea9fd072faa7f5c83eb3 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u3e839ee77504ea9fd072faa7f5c83eb3:hover .ctaButton { background-color: #34495E!important; } .u3e839ee77504ea9fd072faa7f5c83eb3 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u3e839ee77504ea9fd072faa7f5c83eb3 .u3e839ee77504ea9fd072faa7f5c83eb3-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u3e839ee77504ea9fd072faa7f5c83eb3:after { content: ""; display: block; clear: both; } READ: Essay Watching Video Food, Inc. EssayWe can tell that Ma is angry as her husband is not speaking to her, not by Doyledescribing her anger but by the fact that she pays no heed to the pain that thebaby is calling her ? such is the animosity that exists between the couple. Paddy cannot see this, and is wracked by confusion. This is shown a fewparagraphs later: Ma was getting out of the car. It was awkward because ofCatherine. I thought we were all getting out, that it had stopped raining. Butit hadnt. It was lashing. We can see that Ma patience has been tested and,in her ire, she leaves the car. Conformation that Paddy does not understand issealed when he asks ? Has she gone for 99s? His father doesnt reply,the silence filling the void between him and Ma ? unbeknown to Paddy, whoseinnocent question remains unanswered. We are able to read between the lines, andby doing this we can detect the silent turbulence, unlike Paddy whom is thestorys narrator. Paddy Clarke Ha Ha Ha is written in the first person, and istherefore devoid of the authorial omniscience and intrusiveness that would allowDoyle to relate to the reader. The fact that the story is set in a first-personnarrative with a bewildered 10-year old as the narrator allows us to fillthe gaps in P addys mind, and we can connect with Doyles imagination andin doing this he has effectively succeeded in creating a realistic world throughthe eyes of an imaginary child. When reading, the reader and Paddy develop asymbiotic existence, where Paddy is necessary to allow us to see, and hear andact as a viewpoint into his world, and our superior comprehension can observethe underlying tension that ultimately culminates in the parents divorce. Roddy Doyle writes potent novels, rooted in working-class experience. His firstthree novels, known as the Barrytown trilogy, focused on the Rabbittes, a familyof eight whose lives are a mixture of high comedy, depressing poverty anddomestic chaos. The novel Paddy Clarke Ha Ha Ha explores with remarkablesubtlety the development of a small boys empathy, as he simultaneously masterslanguage and discovers a new understanding of pain. Written almost entirely indialogue, his books are full of slang, colloquialisms, and vulgarisms. In thepast, Doyles raw portrayal of working class Ireland has received as muchcensure as praise in his native country. Ive been criticised for the badlanguage in my booksthat Ive given a bad image of the country, saidDoyle. The authors own view is that his job is simply to describe things andpeople as they really are. In Doyles world, the lives are tough, and thelanguage is rough, but beauty and tenderness survive amid the void of bleakness. All quotes are taken directly from the Minerva publication of Paddy Clarke Ha HaHa.

The Queen of the South Review Essay Example

The Queen of the South Review Paper Essay on The Queen of the South life is sometimes so beautiful that it becomes dissimilar to life. » A. Perez-Reverte Teresa was a simple girl Mexican smuggler. Teresa became a fugitive, because she was a girl smuggler who was a great, cheerful and brave guy, but talked too much. We will write a custom essay sample on The Queen of the South Review specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on The Queen of the South Review specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on The Queen of the South Review specifically for you FOR ONLY $16.38 $13.9/page Hire Writer in order to save their skins by drug traffickers, help her to hide in Spain, where it is, how long it seemed, a shelter and a solid shoulder of another man. Another smuggler. Spaniard. But wanting to help a loved and not be in constant expectation of the terrible, she becomes his right hand man. Together they make night raids, but one raid was unsuccessful. And as it turned out the last In prison, she learns a lot, he finds a girlfriend, a meeting which will be pounded to a Mexican fatal.. And that fateful acquaintance will be the first step on the step to the heights of the drug business. In the epigraph I used one of the passages of this novel. Why? Life Teresa was never happy: a terrible childhood in poverty to oppression by the strongest drinking mother, then escape from the killers, the death of loved ones, and at the top, so to speak, of his career the loneliness and . emptiness, fear of being tied to someone, the fear of being out of this vulnerable She was used to that life -. is cruel, merciless and cold Briefly, these burdens were Abort with short segments of happiness in which she saw only a fleeting deceit, knew that soon it kratkotechnoe heat disappear it will take away life . Teresa is respected as the author, and I have. Namely, the desire to be strong, independent and free. She seemed to prove his story to be strong, to be free, so lonely. A solitude is the realm of cold But personally, I do not mind a Mexican She knew that life in this business -.. No sea walk. That life in general a thing is not pleasant, but it has made its choice, knowing the consequences. So she answered him with cold loneliness. The book is written very well, hard to break away from it. A lot informative about how drug trafficking, crime and politics, and the story itself is very exciting. I wanted more of something to read from the Perez-Reverte. And in my case it proves that the book is good:) A quadruple set, because the novel is written (I thought) with a view to further adaptation of, with all the ensuing consequences By the way, the four in my case means that the book is good, but to be read by all is not necessary:)

Free Essays on God In School

The most obvious evidence of school attempts to accommodate people’s religious beliefs and the one thing which is sure to affect everyone involved, regardless of their religious beliefs, is the decision to simply close a school during a religious holiday. Traditionally, this has only occurred around Christmas, but that is starting to change. The question of closing school is a difficult dilemma for school administrators: if they keep schools open, they risk being portrayed as insensitive to the minority religious faiths in their community; but if they close the schools, they risk being portrayed as trying to show favoritism. This, of course, is a consequence of the tradition of always closing for Christmas - if schools never closed for any religious holiday, there could be no charges of favoritism and little basis for the allegation of any particular insensitivity. Unfortunately, that doesn’t mean that schools can simply refuse to close on holidays like Christmas. Sponsored Links Read "Holy War" For A Critical Analysis Of American Christian Fundamentalism www.InquiryAbraham.com Interfaith Alliance US not Christian nation. One Nation, Many Faiths www.interfaithalliance.org Humanist and Pantheist? If you revere the Universe as well as Humanity, you are a Pantheist www.pantheism.net The fact of the matter is, when there are enough followers of a particular religion in a community, you can be sure that on major holidays there will be a high degree of absenteeism in the schools. It might be reasonably argued that schools would be showing hostility towards religion if they didn’t try help students make up for missed work, but it can be easier for schools to simply close and keep everyone at the same stage of instruction. This has been the reason given by school districts when their closing policies have been challenged and the courts have thus far accepted it as a fair and reasonable argument. School closin... Free Essays on God In School Free Essays on God In School The most obvious evidence of school attempts to accommodate people’s religious beliefs and the one thing which is sure to affect everyone involved, regardless of their religious beliefs, is the decision to simply close a school during a religious holiday. Traditionally, this has only occurred around Christmas, but that is starting to change. The question of closing school is a difficult dilemma for school administrators: if they keep schools open, they risk being portrayed as insensitive to the minority religious faiths in their community; but if they close the schools, they risk being portrayed as trying to show favoritism. This, of course, is a consequence of the tradition of always closing for Christmas - if schools never closed for any religious holiday, there could be no charges of favoritism and little basis for the allegation of any particular insensitivity. Unfortunately, that doesn’t mean that schools can simply refuse to close on holidays like Christmas. Sponsored Links Read "Holy War" For A Critical Analysis Of American Christian Fundamentalism www.InquiryAbraham.com Interfaith Alliance US not Christian nation. One Nation, Many Faiths www.interfaithalliance.org Humanist and Pantheist? If you revere the Universe as well as Humanity, you are a Pantheist www.pantheism.net The fact of the matter is, when there are enough followers of a particular religion in a community, you can be sure that on major holidays there will be a high degree of absenteeism in the schools. It might be reasonably argued that schools would be showing hostility towards religion if they didn’t try help students make up for missed work, but it can be easier for schools to simply close and keep everyone at the same stage of instruction. This has been the reason given by school districts when their closing policies have been challenged and the courts have thus far accepted it as a fair and reasonable argument. School closin...

Should liquor ads be allowed on television Essay

Should liquor ads be allowed on television - Essay Example Firstly, most liquor advertisements downplay the factual attributes of the product by focusing on the generation of images and contexts that connote camaraderie, escape, refreshment, and relaxation. Secondly, the youth’s exposure to liquor advertisements often lead to the youth’s heightened interest, trial at young age or increase in frequency and volume of consumption. From a business perspective, advertising is meant to stir the awareness, induce purchase, develop the consumption habit, and build loyalty of the target consumers on the company’s products and services. In the U.S. alone, companies spend nearly $2billion yearly for advertising on both mainstream and digital media, according to a position paper entitled Alcohol Advertising and the Youth. Furthermore, almost 2 million advertising placements on television have been made between 2001 and 2006. Approximately 20% of television alcohol advertisements were on programming that the youth age 12 to 20 were m ore likely to watch than adults of legal drinking age. This finding reinforces the assumption that the companies deem the youth as primary target market of their advertisements. In a similar vein, a briefing paper entitled The Advertising of Alcohol, in support of Increased Restrictions stated that the broadening of communication venues for liquor advertisements through the addition of sponsorship, competition, and special promotions in the brands’ campaign plan underscore the companies’ goal of communicating the relevance of liquor products to the youth.

Resolution of disputes by subcontractors through arbitration in FIDIC Dissertation

Resolution of disputes by subcontractors through arbitration in FIDIC construction - Dissertation Example If there is any delay beyond the deadline, it may cause financial losses to the employer and hence, he may claim damages from the contractor for the losses suffered due to late completion of the construction project. To avoid this , most of the construction contract forms will include a provision for the parties to the contract to agree upon on monthly or weekly or on a daily basis for delay due to the contactor which is known as liquidated and ascertained damages .(LAD). The main objective of LADs is to notify the employer’s prerogative to claim damages for the infringement of a condition by a contractor to finish the work within the deadline1. Unprecedented scenarios are unavoidable element of every construction contract. ... ld† (â€Å"D & B†) or an EPC (Engineering, Procurement and Construction) contract, which is a contractual arrangement thereby imposing the entire accountability for construction and design of the work on a contractor. The phrase â€Å"turnkey â€Å"refers that after the finishing up the work, the contractor is only hand over the key to the owner or the employer to start the operations of the constructed project2. Delays in construction contracts can be classified as excusable or non-excusable. Excusable delay means a delay which may justify an prolongation of performance of contract’s time and some examples of excusable delays are unpredictable weather, changes initiated by the employer, issues with the project designs, acts of god, delays in receiving equipments, which is supplied by the owner, varying or differing work-site conditions, delay due to awarding of contract and labour disputes. If the main contractor is eligible to claim time extension due to unpre dictable weather conditions, subcontractor may be also eligible to claim time extension if the subcontract contains a â€Å"flow down† clause thereby obligating the conditions in the main contract which is automatically applicable to the subcontract also and as it includes an analogues excusable delay provisions3. Under the non-excusable delay, the contractor bears the risk of outcome and cost, not only for himself but also for the resulting effect on the others like subcontractors also. Due to mismanagement, a main contractor may encounter delays, which will also the delay the work done by the subcontractor. In such cases, the main contractor may be accountable to the subcontractors but not to the employer. The notion of non-excusable delay is employed chiefly as a shield to turn down the request for claims for

Faith Community Nursing Spiritual and Health Assessment related to Research Paper

Faith Community Nursing Spiritual and Health Assessment related to Cancer within the Hispanic Faith Community - Research Paper Example faith healing infringe upon early cancer screening, prevention, and / or treatment, as well as how in presence of treatment, individuals still retain their belief in faith healing. The target community was an online community. The target community were 35 – 65 years old Spanish speaking Christian Hispanics. There were 4, 373 members of the blog: 2,500 females and 1,875 males (Graph 2). However, out of them, only 90 females (54% respondents) and 77 males (46% respondents) responded to the survey (Graphs 1 & 2). Respondents came from nineteen different countries: the U.S., Mexico, and several Central and South American countries (Appendix B). Moreover, 25 females and 33 males came from rural areas, and 65 females and 44 males came from urban areas. The survey was distributed on Facebook, where it was posted in a blog, and personal e-mails were sent as well. The survey was distributed under complete confidentiality and anonymity. The target group was highly religious – with a personal relationship to Jesus Christ. All respondents (90 females and 77 males) prayed on a daily basis, read the Bible each week and believed it to be the guide on how to behave, turned to Jesus Christ for everything they did, had a personal relationship to Jesus Christ and believed Jesus Christ would help them with all their problems (see Appendix C). Their sense of community is strong as they all stated they love praying in groups, and go to underprivileged and disrespected communities to preach the Gospel (see Appendix C). However, their health habits are at times inadequate. Participants were split into those with cancer and without. First the latter group will be discussed. Both genders were optimistic that Jesus would save them from cancer: 39 females and 29 males believed so (see Graph 6). As a result, 41 females and 31 males believed they cannot get cancer, and 58 females and 41 males had never been screened for cancer prior to this survey (see Graphs 5 & 4), out of 90

Leadership and Management in Nursing Care Delivery Models

Leadership and Management in Nursing Care Delivery Models A care delivery model is an integral component for delivering patient care. Nursing care delivery model is a way of organizing at the unit level to facilitate the delivery of nursing care to the patients (Tiedeman Lookinland, 2004). Organization of care is a key factor that determines quality of nursing care (Tiedeman Lookinland, 2004). Various types of care delivery models have been designed to meet the goals of efficient and effective nursing care While many different care delivery models are practiced in the wards and departments of the acute care hospital that I work in, it is beyond the scope of this paper to discuss them all. In the 20 bedded medical oncology ward that I work in, nursing care delivery is organised into team and primary nursing. Six beds are allocated to transplant patients. One registered nurse is assigned to be the primary nurse of three transplant patients each. The other fourteen patients are split between two teams of nurses. Each team has a registered nurse, an enrolled nurse and a patient care assistant. Thus, this paper is concerned with these two modes of organization of care. An overview of primary and team nursing models of care will be presented. Thereafter, the experiences of nurses involved in the delivery of care using these models of care will be discussed with regards to job satisfaction and quality of care. Description of Care Delivery Model Primary Team Emerging in the United States in the1960s, the primary nursing model involves patients being allocated to individual nurses (Tiedman Lookinland, 2004). One registered nurse is allocated a number of patients for whose care this nurse is accountable during the patients hospital stay (OConnell, Duke, Bennett, Crawford, Korfiatis, 2006; Tiedman Lookinland, 2004).The nurse-patient relationship is the backbone of primary nursing. The primary nurse collaborates with other health team members, the patient and family to continually assess, plan, implement, and evaluate the patients nursing care (Reed, 1988; Sellick, Russell, Beckmann, 2003; Tiedman Lookinland, 2004). When the primary nurse is unavailable to provide care, associate nurses follow the plan of care developed by the primary nurse, unless a change in patients condition necessitates a modification of the care plan, so as to maintain the continuity of care for the patients (Tiedman Lookinland, 2004). Team Nursing Implemented in the 1940s as a response to the nursing shortage resulting from the World War II, team nursing is based on the premise of collaboration and division of responsibilities for the nursing care of patients (Tiedman Lookinland, 2004). In team nursing, nurses and ancillary staff with different levels of education, skills and licensure are assigned to teams and each team provides total nursing care to the patients assigned to that team (OConnell et at., 2006; Tiedman Lookinland, 2004). Leadership for each team is provided by a team leader, who is an experienced registered nurse responsible for planning and supervising the provision of nursing care for patients cared for by team members. The team leader has overall accountability for coordinating the nursing care provided, while the team members are accountable for completing their assigned tasks and for documenting the care delivered (OConnell et at., 2006). Nursing Staff Job Satisfaction Primary Team One of the most important gains of primary nursing for the nurses interviewed was more contact and enhanced relationships with patients. One nurse said, I know more about each of my patients and do not constantly have to get to know new ones. These views are consistent with Mccleod and Stellas (1992) study where nursing staff on medical and surgical wards were asked what they liked about primary nursing. Participants liked the enhanced relationships and collaboration with patients and other members of the healthcare team. To be able to take care of the same patients day after day was greatly appreciated by the nurses interviewed. This continuity of care led to the development of good relationships between the primary nurse and the patient as well as with relatives. This is an aspect that the nurses found increased their levels of job satisfaction (McCleod Stella, 1992). Primary nursing facilitated an in-depth knowledge of their patients and as a consequence, the nurses felt more able to act as a patients advocate. Nurses verbalised they had additional responsibility and accountability as all duties and responsibilities in taking care of a patient for his or her duration of stay is delegated entirely on a single nurse. The primary nurses interviewed perceived that they had the authority to make clinical judgments and decisions for the benefit of the patient. Their use of phrases such as freedom from control by others, ability to plan and organize the workday, using knowledge to make decisions and advocating for the patient described autonomy. Autonomy refers to the ability to act according to ones knowledge and judgment, providing nursing care within the full scope of practice as defined by existing professional, regulatory, and organizational rules (Gagnon, Bakker, Montgomery, Palkovits, 2010). The nurses noted that through practicing primary nursi ng, their autonomy was increased. There is a substantial body of evidence indicating that nurses perceived autonomy is linked to job satisfaction. A study by Melchior, Halfens, Abu-Saad, Philipsen, van den Berg Grassman (1999) investigated the effects of a primary nursing care delivery system on the work environment among nurses working in long-stay psychiatric care settings. Results showed that as a result of primary nursing, the nurses found more autonomy in their work and thus experienced increased job satisfaction. Similarly, Macguire and Botting (1990) analysed the perceptions of nursing staff, following the introduction of primary nursing into an acute medical ward for elderly people. Nurses reported better communication, improved relationships and increased responsibility after implementing primary nursing. It appeared that both increased autonomy of practice and increased knowledge of specific patients, coupled with a greater continuity of care, gave nurses a high level of satisfaction than did previous meth ods of care delivery (Macguire Botting, 1990). On the downside, some nurses commented that working in a primary care system tends to be isolating and this proved to be particularly difficult when they were looking after a critically ill patient. These experiences mirrored those reported by nurses in the study by Manley, Hamill, and Hanlon (1997). Team Nursing Nurses practicing team nursing commented that working as a team was a lot better, because then they can have somebody else backing them up. According to OConnell et at. (2006), working collaboratively with team members serves to enhance job satisfaction, as many find this to be a learning experience that utilizes the expertise of each team member. This was supported by Rafferty, Ball, and Aiken (2001) who demonstrated that nurses who report a higher level of teamwork are more satisfied with their jobs, plan to stay in them, and are likely to experience less stress. However, enrolled nurses and the patient care assistants interviewed added that they felt the staff nurses were spending too much time with medications and writing reports while they were left to do the heavy manual work. A similar finding was demonstrated by OConnell et al. (2006). In this study, both registered nurses and enrolled nurses felt that the team-nursing model sometimes involved an uneven and unfair division of tasks that overburdened some nurses. The medication responsibilities of registered nurses within the team-nursing model imposed excessive demands on enrolled nurses as they were left with meeting the hygiene needs for the patients in their group without assistance. These tensions led to a decrease in job satisfaction. Quality of Care Primary Team The nurses interviewed felt patients were more satisfied when primary nursing was in place because they received individualized care as a result of continuity and coordination of care. Primary nursing has led to the nurses having a better knowledge of the patient. Nurses pointed out that primary nursing enabled them to know each patient more closely. One nurse remarked, I know my patients like the back of my hand. Therefore, it was easier to find out what each patient needed and to meet those needs appropriately. Jenny and Logan (1992) suggest that greater knowledge of the patient is linked to the increased likelihood of picking up subtle changes in the patients condition. In addition, Perala and Hentinen (1989) found that through primary nursing, doctors received information about patients that was more exact and relevant because nurses knew their patients better. This enabled nurses to provide more applicable and effective patient centred nursing care. In response to this therapeut ic relationship, the patient feels more secure about and satisfied with nursing care (Jenny Logan, 2000). The nurses views were supported by the findings of Radwin (2000). In this study conducted over a 3-year period, oncology patients were asked to identify qualities of nursing care that were important to them. Professional knowledge, joint decision making between the patient and the nurse, individualized treatment plans, promptness to addressing individualized needs and continuity of care were some aspects of care delivery that were identified. Team Nursing Nurses interviewed said that since staff was supervised more closely, the likelihood of missing things out was low. This, they felt, resulted in patients receiving better care. This sentiment was echoed by the nurses in OConnell et al.s (2006) study. Moreover, nurses added that the patients benefitted from the combined skills of the team. According to the study by Cioffi and Ferguson (2009), team nursing made a difference to patient care as it was patient-oriented, all the nurses in the team were familiar with the patients receiving care, care was more complete as things were missed less often and staffs were supervised more closely. This resulted in patients receiving better care. In conclusion, providing care to a group of patients require nurses to be more efficient and use their time more effectively. Nursing care delivery models facilitate this. This paper has provided an overview of primary and team nursing care delivery models. The experiences of nurses were discussed with regards to job satisfaction and quality of care in the two models. Literature supporting the nurses experiences was then explored.

Educating Tomorrow’s Generation Essay examples -- Philosophy Education

Educating Tomorrow’s Generation Education is defined as the act or process of educating a person. With that in mind, what is the best way to go about providing an education for tomorrow’s generation? First, the way to educate a person is to teach them. The purpose of education is to provide students with the knowledge they will need to have a successful future. When it comes to the way to educate kids I agree with John Dewey and Progressivism, which basically says we are suppose to assist with the growth of individuals. Education is one of the most important things for young people growing up, because it is their ticket to a better life. After reading all the different types of philosophy, I have decided that I agree with progressivism. The man most responsible for progressivism is John Dewey. His main point in starting this progressive schools was to offer students with a broaden curricula. This put education more to the needs of the students and made school more enjoyable as well. I think these are two very important points with education. One of my main goals as ...

Perks of Being a Wallflower

â€Å"And all the books you’ve read have been read by other people. And all the songs you’ve loved have been heard by other people. And that girl that’s pretty to you is pretty to other people. And you know that if you looked at these facts when you were happy, you would feel great because you are describing â€Å"unity. † (p. 96). â€Å"The Perks of Being a Wallflower† written by Stephen Chbosky was published by MTV books and Pocket Books on February 1st, 1999. It’s classified as Young Adult, Contemporary Fiction and Epistolary novel. Epistolary novel is a novel written as a series of documents.Anne Frank is a type of Epistolary novel. Unlike Anne Frank, this one is written in form of letters, not a diary. In this novel you can see how â€Å"Charlie’s† life changes throughout his freshman year. How he deals with all the problems he faced, he faces, and he’ll face. In a unknown setting, the novel begins August 1991 with a teenage boy going by the alias â€Å"Charlie†, writing to an anonymous friend. â€Å"I just need to know that someone out there listens and understands and doesn't try to sleep with people even if they could have.I need to know that these people exist. † (p. 2). He heard someone at school talking about this anonymous friend, and he thought it would be someone nice to talk to. He specifically asks this friend to not try to find his true identity. Charlie has begun his freshman year while his brother is at Pennsylvania State University and his sister is at her senior year. We learn that his best friend, and only friend, committed suicide before he started writing these letters, leaving Charlie alone in high school.His favorite person in the world, his aunt, also died when he was 7 years old. He states repeatedly that something bad happened to her, but he doesn’t mention what, because it brings him to a bad place. After she died he doesn’t remember his lif e for a year, and he had been â€Å"different† ever since. As he starts freshman year, he is an outcast, until he meets Sam and Patrick. They introduce him to an electric, open-minded, hard-partying life, and soon Charlie starts enjoying his life. â€Å"And at that moment, I swear we were infinite. † (p. 39).Experiences that Charlie and his family and friends go through and the topics explored throughout the novel include suicide, difficult/abusive relationships, drug use/smoking, sex, abortion, child abuse/trauma, the struggles of homosexuality, and the awkward times of adolescence, such as first kisses and first girlfriends. Charlie is a troubled teenage boy. He is insecure and shy, because he just lost his best friend, and doesn’t know to deal with school alone. Since the death of his aunt, which he takes the blame, [â€Å"Despite everything my mom and doctor and dad have said to me about blame, I can't stop thinking what I know.And I know that my aunt Helen would still be alive today if she just bought me one present like everyone else. † (p. 92)], he has been different, sort of out of sync. He’s very sentimental, and he analyzes everything too much. Every tiny detail, is a big deal for him. He loves music and literature. His teacher gives him books to read and write bout, such as To Kill a Mocking Bird. His aunt molested him at the age of 7, but he never told anyone, because he loved so very much.

Hygiene and Young People

‘Your health, safety and welfare are protected by law.’ Everyone working with children and young people must be aware of their legal obligation in relation to keep children safe and healthy. (Source: Health & Safety Executive, 1999)Health and safety legislation at work Act 1974:Setting with five or more employees must have a written safety policy which must include specific procedure to cover emergencies such as accidents and events that require evacuation of the building (Fire event) Setting with five or more employees must carry out a risk assessment Employees must provide for health and safetyDisplay a health and safety laws poster or supply employees with a booklet Make your work place as safe as possibleDecide how to manage health and safety if the business has five or more employees COSHH (Control of Substances Hazardous to Health Regulations 2002): The employers are required to ensure all dangerous materials, cleaning material, medicines are kept separately in a m arked, locked cupboard. RIDDOR (Reporting injuries, Diseases and Dangerous Occurrences Regulations 1995): This means all setting must provide an accident report book. Any injury that requires a member of staff to take more than three days off work must also be reported. Food safety (General Food Hygiene) Regulations 1995:The setting which prepare or provide food for children must register with the Environmental Health Department of the local authority. The people who have handling food should undertake the Basic Food Hygiene Certificate. Food handling Regulations 1995Washing hands before preparing foodMaking sure the surfaces and utensils you use are clean and hygieneFood is  stored safety at the correct temperatureDisposing of waste hygienicallyUse of chopping boards, separate sinksPolicies and Procedure:Children and young people will always take a risk from running, playing but all children and young people have right to play to develop their skills and new experience. There are policies and procedure to protect and reduce risk that will happen to children and young people. Every child care setting will have policy documents which cover SafetyHealth and Hygiene Safety at arrival/departure times and on outings Prevention of illness and first aid Fire prevention Staffing ratios and supervision Risk Assessments Policy.A large number of everyday things that may appear harmless or go unnoticed but could be dangerous A range of areas from the condition of toys and equipment to hygiene and cleanliness to access to children by members of the public Must be suitable, sufficient and reasonably practicalAccording to legislation, policy and procedure as showing above, in my setting (school placement) they are implemented by the school has a fire exit at the front door. There are several risk assessments for several areas in school. At the front door, there is a registered book for staffs and visitors to sign time in and out and purpose of visiting. After lunch time, f ood area has been clean and tidy up all tables and chairs to keep children safe. All toys have been kept in place and safe, study resource and materials are kept in cupboard. There are sinks in classrooms for washing hands.2. Describe how health and safety is monitored and maintain in the setting. Health and safety is monitored and maintain in the setting by: Visitors register book, record time in/out, purpose and who they want to meet Teacher  and all member of Staffs register book, teacher and all members record time in/out Pupils register book, checking pupils in class, morning and afternoon Lunch time staff, monitor pupils at food areaTeacher/member of staff at the school gate, morning and after school Teacher/member of staff at the playground and play area every break times3. Describe how people in the setting are made aware of risks and hazards and encouraged to work safely. In the setting, teacher and all members of staff, volunteer must be made aware of risks and hazards a nd encouraged which is in school health and safety policy. The school role and Staff handbook are written by head teacher in the setting, staff handbook is held by teacher each class.Risks on internet in the setting is controlled by teacher, if pupils in class access on Facebook, YouTube and etc. websites that may be harm to pupils, teacher able to switch off computer immediately from teacher’s desk. To protect bullying, there is anti-bullying poster throughout school, poster show how to protect and prevent risks on internet includes the children help line number.Fire is the most frighten pupils and may harm their life, the setting made aware of this risk assessment, pupils can follow fire instruction poster which is throughout school when fire alarm appear. In this situation, teacher will lead pupils to fire exit door which is located at every classroom to school yard and line up at the meeting point. Pupils must be registered by their teacher at the meeting point.The meetin g among Head teacher, member of staff and parents after school time will be made 2-3 times a year, the meeting will be discussed how to solve problem and sharing information between school and parents.4. Identify the lines of responsibility and reporting for health and safety within the setting. Head Teacher is in charge for accident events, Food Hygiene and Head Teacher’s secretary ensure a risk assessment has been following by staffs who work in the school. By the way all staffs who work in the school have been trained to keep children safe and reduce harm in the  school.

Summary Sample on International Business in the New World Environment

Summary Sample on International Business in the New World Environment There was a time when travelling around the world in 80 days symbolized a great feat in travel and exploration. Today, travelling the world in a day is becoming as common as buying a loaf of bread. As our world become smaller, businesses find that they must adapt to the new world economy and environment. For Multi-national enterprises (MNE)s this poses a challenge to be ahead of the game when it comes to being competitive. More specifically, MNEs must decide how to progress their agendas for the future world of business through their Human Resources Management (HRM) policies, which plays a critical role in a businesses success. Businesses face a new age of understanding global dynamics. Knowing how to develop strategies that align with all corporate strategies to be able to compete with an ever increasing and complex process of securing value resources. HRM is key to a business success. Companies who strategically link their human value tend to be more successful than companies who do not. This is due to the fact that companies managing international careers can face obstacles that include cultural, economic, political and economic differences which are to be considered in operational decision making. HRMs ultimate goal is to recruit, develop and retain the best managers. If this is to happen various factors must be thought out that are interlinked with success. Companies who understand this important fact display evidence of this in areas of their business like in shareholder value. That being said, developing a good HRM strategy and implementing one is a different matter. The reality is that companies dont always implement the HRM strategies as communicated. Studies show that managers tend to leave companies after resettling and companies themselves report HRM is their weakest point in their business. To bridge the gap of HRM and the reality of it, companies need to make it an agenda or action plan. The alignment of HRM strategies with all operations is key. MNEs need to consider whether they use local, citizen, non-citizen or expatriates employees. A companys values and ideas translate into assumptions or generalizations called frameworks. MNEs apply these frameworks to their staffing policies via in three different types of frameworks. The ethnocentric approach is based on the belief in the supremacy of the home HRM. Being based in the core competencies of the managers, it can blind HRM policies to country difference and dulls competitiveness. The polycentric approach is a HRN policy that is country specific. This approach usually provides good use of local managers although the responsibility transfers to new company and disengagement of local staff with international offices can occur. Thirdly, the geocentric approach looks for best candidates globally. Though a hard and costly endeavour to develop it is also an approach where the gap between reality and theory is big and it is thought to be impossible to manage. Normally when companies decide to send expatriates to set a new branch or subsidiary they would have a team that has a finance person, a person with good entrepreneurial skills and a line manager with excellent soft skills. These skills seem to cover the range of knowledge and expertise needed to overcome cultural, economic and legal issues. However, a major decision lies in the choice of expatriate candidate. A good expatriate candidate possesses the qualities of technical expertise, adaptive skills and leadership abilities. Nonetheless, studies show that 70% of the best managers sent abroad still failed. The biggest problem to success is how the spouse of the expatriate handles the move. To solve the problem companies develop a preparation and development program, which includes general country understanding, cultural sensitivity, and practical skills, training gaps and skills (i.e. salary differences). For the expatriate themselves, they too have a range of compensation plans on potentially available to them. These include home-based plans, headquarters based plans, host- based plans. Included in these are the salary plans for expatriates, which are comprised of a combination of base salary, allowances (which can include tax benefit and fringe benefit such as medical coverage) and Foreign Service premiums. Similarly, building an organization to implement a strategy is difficult. Consideration has to be made with various different ways to address issues such as information systems, human resources, etc. Traditionally companies build systems using formal structures. Nowadays, there are two trends emerging. Environmental refers to the diffusing of international business and globalization. Then there is the organizational trend that refers the growing knowledge competition businesses are confronted with. How a company chooses to structure itself is also essential. A company’s structure can take the form of a centralized or decentralized. Determining where the hierarchy begins is what determines the formal structure. Decentralized are usually multidomestic strategies while centralized is more international strategy and is designed in a horizontal differentiation. Horizontal differentiation is how a company to specific tasks and divide those tasks and assign authority to accomplish company strategy. Within a decentralized or centralized structure a company can choose from various structures, which include functional, divisional, international division, product division, geographic division structure. A matrix structure incorporates a system of reporting to several groups to accomplish a companys structure. Challenges of matrix structure include competing division, dual hierarchy. The previous structures are difficult to recreate in reality that leaves company in a sort of mixed structure that deals with functional, area and product dimensions. Nonetheless, all these are considered to be traditional structures. Contemporary structures are evolving. They exist due to the decrease of the boundaries of business and examples can be a virtual organization, modular structure or learning organization. Also, a network structure is now emerging were managers outsource value activities. This strategy allows companies to do what they do best and get the expertise of other companies who specialise in their fields to compliment their activities. An interesting development is virtual organizations is that these organisations rely heavily on technology to share resources. Similarly, the project structure of business operates based solely on projects and disband after projects are complete. Although highly innovative this structure has been difficult to sustain. Value chains require coordination systems to mange performance. This can be done through coordination by standardization, which are not flexible, coordination by plan, which gives interdependent units the flexibility to adapt to variations and the coordination by mutual adjustment, which allows managers to adjust decision-making. Supporting these coordination systems are control systems that deal with how employees implement elements planned by a company. Affecting these systems are types of controls. There are market controls that establish benchmarks based on markets. Next you have bureaucratic controls that are a set of rules set up be centralized authorities. Then you can have clan controls, which is the realization of employees about shared ideas of the company. To complete a companys system of control they can implement various types of control mechanisms. These mechanisms direct a company’s activities. They include the use of reports and information technology (such as ERP enterprise resource planning) can be major factor to consider especially with geographic distance. Similarly, companies can opt to visits to subsidiaries to collect information and offer advice directly. Or a company can use management performance evaluations, cost and accounting comparability mechanism, evaluative measures and/or varying information systems. All of these components can drive the strategies of a company based on it structure. Finally, not only must a company address culturally issue outwardly but they must focus on their own internal cultural structures. A business organizational culture is vital. The organizational culture is basically the fundamental assumptions members of a company share. This is an ever-increasing topic to be concerned with as companies relate to global competitiveness. In addition, the challenge of managing culture changes should not be reactive if they are to triumph in this theme. Some companies try to have closer contact with managers in different countries or rotate managers from country to country in an attempt keep managers abreast of cultural issues. Companies must pull out all the stops for victory.

What Coding Means for Reimbursement in a Physicians Office Essay

What Coding Means for Reimbursement in a Physicians Office - Essay Example This process entails building of patient’s medical records whilst under the care of the practitioners. The medical coders must adhere to the correct procedures of coding to come up with standardized codes, which are recognizable by the insurance companies. Having this information easily identifies the correct codes that are used, which are universal. Proper coding will indeed reduce any events of failure that may result from coding. Buck is of the opinion that medical coding, in this case, is needed to ensure that proper checks are conducted3 and thus to make it easier for the insurance companies to review and assess the patients’ claims. Medical coders ensure that all blood work and diagnostics performed by medical doctors consist of the required coding and are correct and standardized. This relates to the fact that medical coding allows for the use of the correct codes and data and, consequently, effectual review of all claims that are submitted4. The set codes are then used to encode all claims that health insurers receive. In the laboratory, medical coding has played the role of reviewing the tests that medical practitioners prepare to conduct. Through coding, doctors are able to carefully assess the tests and the help of medical coders. Close collaboration between medical coders and doctors makes it easier to double-check the entire process in case of any complications in the paperwork. Additionally, this coding process through team work ensures that there are minimal delays in the payments made whilst in the laboratory. Time is also well spent in this process of review, which is of great use in the medical world. It can be concluded that medical coding is a process that should be undertaken with utmost deference by all medical practitioners if a small number of complications and cases are to be characteristic of any medical facility. All

South Korea Market Assignment Example | Topics and Well Written Essays - 1000 words

South Korea Market - Assignment Example Through planning, direct or indirect ownership and control of enterprises and financial institutions, regulation of foreign exchange, and implementation of appropriate monetary and fiscal policies, the Government played a crucial role in making market adjustments and maximizing incentives. This was aimed at pursuing the fulfillment of its desired economic, social, political, and cultural objectives. However, economic success, the increase in complexity of the economy, the emergence of a more democratic and pluralistic society, and increasing international competitiveness were crucial factors in bringing about a decline in governments’ role in the South Korean economy. The reformist thrust of government economic policy, realized in the 1980s, was underlined by the renowned emphasis on the need for greater efficiency in the economy. In addition, it improved labor productivity and enhanced competitiveness in order to meet the demands of globalization. The admission of South Korea , in December 1996, to membership of the Organization for Economic Co-operation and Development (OECD) was another watershed, which heightened the importance of economic reform, which is a step away from negativity – the Asian financial crisis, which highlighted long-standing structural weaknesses in the South Korean economy (Paik 69). Demographic Information of South Korea South Korea is, in fact, regarded as one of the world’s most densely populated countries, with 440 people per square kilometer. However, most people are concentrated in the major cities (Lie 16). The principal cities in South Korea include Seoul, Busan, Daegu, Ulsan, and Incheon. In these four cities, the population density is about two-thirds of the whole population of South Korea (Caselli 129). The country’s capital city is Seoul. It covers about 12% of the total country’s area. Like the other cities, it is home to approximately 45% of the country’s population; as such, the 3r d largest and populated urban center in the world. Current statistics show that more than 50% of the immigrants are moving to the capital city. Indeed, it has been speculated that about 55% of the national population will be living in the capital city. South Korea is officially recognized as the Republic of Korea with an area of 99,268 km2. According to information published by the World Bank, the total population is 48.747 million by 2009. Figure I. Republic of South Korea (Source: U.S. Department par. 1) The official language in the region is Korean, and it is closely related to Mongolian and Japanese. Although there is a clear difference in grammar with the Chinese language, a large number of the Chinese cognates still exist in the Korean language. Modern Korean articulates to hangul—a phonetic writing system—in their writings. The country borders North Korea on its Northern part. The country is also surrounded by a mass of water encompassing the Sea of Japan and th e Yellow Sea. It is separated from Japan by the Sea of Japan (Paik 77). Korea’s ethnical group has been regarded as the most linguistically homogenous in the whole world. The region is occupied by Koreans, however, about 20,000 people of Chinese descendants occupy some parts of the country. Indeed, all Koreans have a common linguistic and cultural heritage. The South Korean political system is based on the Republican form of governance. The President is the Head of State, and the Prime Minister acts as the Head of Government. The power is shared between the legislature, executive, and judiciary. Currently, the South Korean president is Lee Myung-bak, while the Prime Minister is Han Seung-soo.

A Portable Printer and Scanner Assignment Example | Topics and Well Written Essays - 1000 words

A Portable Printer and Scanner - Assignment Example This innovation is occurring through experimentation with new approaches to market development in emerging markets. It is important to reposition this product attracting a wider customer audience often omitted in advertising. High-tech global consumer positioning works well for special interest products associated with prefessional sphere. Since most mobiel professionasl and students who buy and use high-tech products speak the same language and share the same mindset, marketing communications of JetLux will be informative and emphasize performance-related attributes and features. The target group for the Pentax PocketJet will be devided into two sub-groups: people who work at home; small offices (2-3 people). A portable printer JetLux not only allows this group to save time, but it helps to reduce spending on calls to the office and having to wait for someone print the documents. This allows Pentax: to shift its focus to brand image and price reduction measures. to maintained high-speed growth. establish trustworthiness, confidence, and competence for customers. The Impact of Online and Offline Positioning, and Buyers Respond The aim of the message is to appeal to consumers' mind with unique selling proposition. Online and off line promotion will inform potential customers about a new product. This product represents boundless opportunities for marketing campaign within flexible geographical boundaries. Offline buyers 1. Many customers do not have time to read press or listen to the radio, so in-store demonstrations will be the main tool to attract new customers. 2. JetLux will be promoted through computer and mobile stores, based on the fact that there is a strong link between the printer and PC businesses. 3. This target group will involve people who have to work outside of the office and need a high quality printing a low price. 4. It is expected that some customers will buy spontaneously influenced by product demonstrations and evident product benefits. 5. Some of the buyers will go online searching for detailed information and product features. Online buyers 1. The role of Web site is to inform the market clearly and persuasively about the company and its product characteristics. 2. Most buyers are driven by success and brand prestige and brand loyalty. Online buyers value good technology which allows to

Simple Gift Essay Example for Free

Simple Gift Essay The novel ‘the simple gift’ is written by Steven Herrick. He describes three main characters in this book. This book is about one 16 year old boy called Billy, he run away from his house and meets other main characters, Old Bill and Caitlin. Each of there characters received a gifts. Billy is a 16 year old boy. In Billy’s life his father is an alcoholic and is very mean to him. Billy always wants someone who looks after him and then he can learn how to care. This became true when he runs away from his father and meets Old Bill. Old Bill is like a loving father figure to him. So he considered the friendship with Old Bill as a gift. When he stays in the carriage he felt that this is the home for him and Old Bill is his only family. Old Bill is an alcoholic man. He lost his wife and his daughter Jessie. Which makes him a hopeless man and he started drinking to getaway for the suffering (pg.95~98)†I lifted the glass and downed it in tone ignorant gulp and I called for another as all thoughts of truth and beauty washed from my mind†. Old Bill lives in a carriage. Even though he has his own house with a big backyard but he still doesn’t want to live in the house with no one else but himself living in it, with no one else care about him, even himself. This has changed when he met Billy. Every morning Billy brought him breakfast with a bowl of milk and Weet-Bix (pg.76)† Every morning that kid has woken me with Weet-Bix.†, spent time talking with him, and Billy also tried to stop him from drinking heavily. (pg.77, 136)† This kid’s going to turn me into a health. He brought out the ginger beer I swore and laughed.† Caitlin is nearly 18 year old. She works at McDonald’s as a part time hob, to get out of her house (pg.36~37)†I can leave home and that’s why I work at McDonald’s and mop floors.† She’s rich, she had everything she wants but she needs a lover (pg.88)† I know what I really need and it’s not in my bedroom. And it’s not able to be bought in any damn store†. She wants to have a good time with someone she likes. When she met Billy, she had a romantic love experience. They shared food with each other and when she had a lover, her life was perfect (pg.135)†what can I say, it was like stepping into heaven, no less than perfect†. Steven Herrick describes three main characters. Characters offered each other gifts and their gifts had changed each other’s future and are making their life perfect.

Method Development for Protein Detection

Method Development for Protein Detection Binding-induced and label-free colorimetric method for protein detection based on binding-induced DNA hybridization and DNAzymebased signal amplification INTRODUCTION In diagnosing the early stage of a disease or pathological condition, proteins, especially those associated with cancers, are of great importance because they are the molecular machinery of life.[BB2013-AM-3, 1,2] Enzyme-linked immunosorbent assay (ELISA) is the most commonly used method for protein detection.[PD-3,1,2] Unfortunately, this antibody-based assay requires a long incubation periods and long assay times with the involvement of multiple washing steps.[PD-5,1,2] Additionally, it is faced with the challenges of insufficient sensitivity and limited dynamic range.[PD-4, 5,6,7] As an alternative to the antibody-based assay, aptamer-based assays have gained tremendous attention recently.[PD-4,9-11] Aptamers are single stranded DNA or RNA oligonucleotides selected from random sequence nucleic acid libraries through an in vitro selection process termed systematic evolution of ligands by exponential enrichment (SELEX).[PD-6,4-6] They possess high affinity and good selectivity for s mall molecules, proteins or other targets.[BB-11,20-22] Compared to antibodies, aptamers exhibit obvious advantages including a better stability for long-term storage, a rapider preparation by chemical synthesis in large quantity, and the flexible modification with a variety of functional groups.[PD-4, BB-11,23,24] Some aptamer-based amplified detection assays for protein have been developed in the past two decades, such as the polymerase chain reaction (PCR), rolling circle amplification (RCA), strand displacement amplification (SDA) and ligase chain reaction (LCR). [BB-AM-3] Although these amplified assays greatly enhance the sensitivity of protein detection, they are usually time-consuming and too complicated. Therefore, the amplified detection of protein is still challenging in bioanalytical chemistry.[BB2013-AM-3] Deoxyribozymes (DNAzymes) are artificial nucleic acids, which are isolated from in vitro selection. [DM-5] Similar to traditional protein enzymes, they exhibit high catalytic hydrolytic toward specific substrates, while they possess higher thermal stability that can be denatured and renatured formanycycles without losing catalytic activities.[DM-4] This obvious advantage makes DNAzymes ideal biocatalysts for achieving signal amplification in biological applications.[DM-4] An important development in the DNAzyme field is the discovery of the G-quadruplex DNAzyme.[DM-9,9] The G-quadruplex sequences can associate with a cofactor, hemin, to form peroxidase-mimicking DNAzymes to catalyze the H2O2-mediated oxidation of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to a green-colored product ABTS†¢Ã¢Ë†â€™ or enhance the chemiluminescence of the luminol-H2O2 system.[DM-6,25, DM-5,44] With this main advantage, G-quadruplex DNAzyme has been employed to develop many colorime tric, chemiluminescent or fluorescent sensing platforms for the detection of proteins, DNA and other biomolecules.[DM-9,14,DM-2,34-37] Recently, Willners group reported an enzyme-free amplified detection platform based on the hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme.[AC2012-2] This strategy is quite successful, while the target detection is limited to DNA, and the detection of protein represents another challenge. In this work, taking the advantages of the high selectivity and affinity of aptamers and the HRP-mimicking DNAzyme amplification strategy, we designed a new binding-induced and label-free ultrasensitive colorimetric method for amplified detection of protein. As a proof of principle, human ÃŽ ±-thrombin and its two aptamers, Apt29 and Apt15, are used. In this sensing system, Apt29 and Apt15 are integrated into the proximity probes as recognition elements for the thrombin. These two proximity probes hybridize with each other stably only when both of them bind to the thrombin simultaneously.[BB2013-AM-3] The binding-induced hybridization duplex triggers an autonomous cross-opening of the two functional hairpin structures. And this leads to the formation of a variety of hemin/G-quadruplex DNAzymes. The DNAzymes catalyze the oxidation of ABTS, generating a green colorimetric signal, which can be monitored simply by a spectrophotometer. [DM-3,29,30] This binding-induced and DNAzyme-based s ignal amplified method has a great potential for protein detection. [BB2013-AM-3] In addition, since various recognition elements might be fused, this method can be further extended to sensitive detection of other proteins.[DM-4] EXPERIMENTAL SCETION Materials and Reagents. All DNA oligonucleotides were purchased from Genscript (Jiangsu, China). The oligonucleotides were PAGE-purified and diluted in pH 7.4, 20 mM Tris-HCl buffer solution (containing 100 mM NaCl, 20 mM KCl, and 2 mM MgCl2) to give stock solutions of 100 ÃŽ ¼M. Before use, two hairpin structures were heated to 95à ¢Ã¢â‚¬Å¾Ã†â€™ for 5 min, and slowly cooled down to room temperature. Human ÃŽ ±-thrombin (Tb), bovine serum albumin (BSA), and human serum albumin (HSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hemin, [tris(hydroxymethy-l)aminomethane] (Tris), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), 2,2-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), and H2O2 were purchased from Aladdin Reagents (Shanghai, China). A hemin stock solution (1 mM) was prepared in dimethyl sulfoxide (DMSO) and stored in the dark at −20  °C. All other chemicals were of analytical grade and were used without further purification. All solutions were prepa red using double-distilled water, which was obtained through a Milli-Q purification system (Billerica, MA, USA). Absorbance Measurements. Absorbance measurements were performed under room temperature using a TU-1901 UV−visible spectrophotometer (Beijing Purkinje General Instrument Co, Ltd., China). Kinetic data were recorded at the wavelength of 420 nm every 5 s during the first 5 min of the reaction. The absorption spectra of the solution was measured in the wavelength range from 390 to 490 nm. Procedure for Thrombin Assay. The experiments were performed in 50 ÃŽ ¼L of Tris-HCl buffer (20 mM Tris-HCl, pH = 7.4, 100 mM NaCl, 20 mM KCl, 1 mM MgCl2) containing 200 nM P1, 200 nM P2 and varying concentrations of Tb. The mixture was first incubated for 30 min at room temperature to allow complete binding. Next, 25 ÃŽ ¼L of 2 ÃŽ ¼M H1 and 25 ÃŽ ¼L of 2 ÃŽ ¼M H2 were added and incubated for 6 h at room temperature. Then, 20 ÃŽ ¼L of 2 ÃŽ ¼M hemin and 240 ÃŽ ¼L of HEPES buffer (25 mM HEPES, pH= 7.4, 200 mM NaCl, 20 mM KCl, 0.05% Triton X-100, 1% DMSO) were added, and allowed to incubate for 1 h at room temperature. Finally, 30 ÃŽ ¼L of ABTS and 10 ÃŽ ¼L of H2O2 were added to the mixture to give the final concentrations of 2 mM and 2 mM, respectively. The resulting samples were tested with a UV−vis spectrometer. RESULT AND DISCUSSION Design strategy for human ÃŽ ±-thrombin detection The sequences of the oligonucleotides used in this work were listed in Table 1. It consists of two proximity probes (P1 and P2) and two hairpin structures (H1 and H2). Both proximity probes P1 and P2 consist of four domains. Domain I includes two different thrombin aptamers, Apt29 (29 mer) and Apt15 (15 mer). The Apt29, orange domain of P1, binds to the heparin-binding site and the Apt15, skyblue domain of P2, binds to the fibrinogen-binding site of thrombin, resulting in proximity. Domain II (black) consists of a poly-T sequence that is designed to reduce the effect of steric hindrance induced by thrombin. Domain III (pink) is designed to have only 6 complementary bases, so that two proximity probes P1 and P2 cannot form a stable duplex without the target protein at room temperature. Domain IV (blue) is the key domain for binding-induced DNAzyme-assised signal amplification. By using two functional hairpin structures, the recognition of domain IV could trigger-on the hybridization c hain reaction that led to DNAzyme chains consists of the hemin/G-quadruplex HRP-mimicking DNAzyme. Hairpin structure H1 is functionalized at its 5 end with three-fourths of the G-quadruplex sequence, domain V (green), which is linked to the programmed sequences VIII (red) and VII (blue). One-fourth of the G-quadruplex sequence, domain VI (green), is extended at the 3 end of the hairpin H1. Hairpin structure H2 is functionalized at its 5 and 3 ends with one-fourth of the G-quadruplex (domain VI) and three-fourths of the G-quadruplex (domain V) sequence, respectively. Programmed sequences of domains VII and VIII in hairpin H2 are complementary to domains VII and VIII in hairpin H1, respectively. Both four domains in hairpin H1 and H2 are incorporated into a stable hairpin configuration in an initially locked format by hybridizing with their partially complementary sequences. It is noteworthy that sequence V is partially hybridized with domain VII in hairpin H1 or VIII in hairpin H2, w hich prevents the self-assembly of the active hemin/G-quadruplex DNAzyme. Principle of binding-induced DNAzyme-assisted amplification strategy for human ÃŽ ±-thrombin detection The working principle of human ÃŽ ±-thrombin detection is illustrated in Scheme 1. In the absence of thrombin, domain III in P1 and domain III in P2 will not associate since the complementary sequences (6 nt) are too short to promote efficient hybridization. When the target thrombin is introduced into the system, domain I in P1 and P2 bind to the protein simultaneously, resulting in domain III and III sufficiently close and to hybridize to each other to form a stable P1-Tb-P2 duplex, step 1. [BB2013-AM-3] Once the P1-Tb-P2 duplex forms, it associates with the stem region of H1, domain VII, leading to an opening of H1. This opening of H1 results in the release of the single-stranded domain VIII and the conserved three-fourths of the G-quadruplex (domain V), step 2. The released domain VIII then hybridizes with domain VIII of the stem in H2, and opens H2 using the strand displacement principle, step3. Subsequently, the liberated domain VII in H2 cross-hybridizes with H1 by hybridizatio n of domain VII to domain VII in H1, resulting in two G-quadruplex subunits (domain V and VI) sufficiently close and to self-assemble into a G-quadruplex structure, step 4. [AC2012-4] In the process of this autonomous cross-opening of H2 and H1, strand displacement can be repeated continuously, generating numerous G-quadruplex structures. In the presence of hemin, the resulting catalytic hemin/G-quadruplex peroxidase-mimicking DNAzymes catalyze the H2O2-mediated oxidation of the colorless ABTS2− to green-colored ABTS†¢Ã¢Ë†â€™. Detection of thrombin in human serum To further demonstrate the feasibility of the proposed method in real bioenviroments, we performed the detection of thrombin in human serum. [AC2013-4] Three concentrations of thrombin (10 pM, 100 pM, and 1000 pM) were spiked into 10-fold diluted human serum. [ZK-CC-1] Figure 4 shows the time-dependent absorbance changes of ABTS†¢Ã¢Ë†â€™ in response to different concentrations of thrombin. [AC2014-6] In logarithmic scales, the absorbance value exhibits a linear correlation with thrombin concentration over a range of 3 orders of magnitude from 10 pM to 1000 pM (inset of Figure 4B). [AC2012-12] The result indicated the potentiality of the proposed method for protein detection in real biological samples. [AC2013-4] Conclusions In conclusion, we have developed a binding-induced and label-free colorimetric method for protein detection based on binding-induced DNA hybridization and DNAzyme-assisted signal amplification. This method does not require any modification of DNA and involve any protein enzyme, which makes it technically label-free, enzyme-free and very cost-effective. Furthermore, the present approach uses a simple separation-free procedure in which the assay is conducted in a homogeneous solution.[AC2014-3] In addition, due to the excellent specificity of two proximity probes to the thrombin and the ingenious design of two hairpin structures,[AC-EA-2] this method exhibits a high sensitivity for thrombin detection, with a low detection limit of 2.5 pM. More importantly, this method can be extended to sensitive detection of other proteins by simple changing the aptamer sequences of the two proximity probes. To sum up, this simple and cost-effective colorimetric signal amplified method has great poten tial to be used as a universal tool for ultrasensitive analysis of thrombin or other proteins in serum and supply valuable information for biomedical research and clinical diagnosis.[ZK-CC-1]

Life in the 90s Essay -- essays research papers

Throughout the 1990’s there was a lot of good and bad times. Starting with the good things, I will begin my paper with 5 of the up-most important events in this period of time and then conclude with 4 of the down sides. Early in the 90’s was Desert Storm, which was a huge success. The 1991 bombing of Baghdad was a demonstration of smart, powerful weapons, planning ahead and military superiority. Having control in the air was a major goal of those who had planned out the attack. Starting attacks on the capital was the major idea and political goal of destabilizing Saddam Hussein's regime (basically the main purpose here was to have Iraq’s leader murdered). About 244 laser-guided bombs and 88 cruise missiles were delivered against Baghdad targets in 43 days of war. That’s most of the powerful weapons used in Desert Storm. Overall, around 250,000 individual bombs and missiles were dropped or fired in the 42 days. There were only 12 days and 12 nights when there were no strikes against Baghdad. There were only 14 nights when more than two targets were attacked within the city. For a lot of reasons, including growing political constraints on capital missions, fewer Baghdad targets were bombed as the war progressed. The campaign's end contrasted right after the opening night display. I think that Desert Storm could thank the war planners for the high achievements because they way they attacked Baghdad was almost like a sneak attack, and hey weren’t prepared, Overall, they were caught off guard. Next I will move onto wireless communication.   Ã‚  Ã‚  Ã‚  Ã‚  Now a new â€Å"fad† in the 90’s is things such as cellular telephones, everyone has them. Behind the calling process is more than you know before you purchase these items. When a cellular phone makes a call, it normally transmits it's a certain wave out to a cell phone site (towers). The number called in a short overwhelming piece of data. This data is the short buzz you hear after you hit the SEND button and before the tower catches the data. These things are the components the cellular provider uses to make sure that the phone is programmed so that it is billed and that it also has the identity of both the customer and the phone. Now kids starting at almost the age of 13 begin to carry these items. These are a high point of technology in the 90’s and everywhere you tur... .... Some schools districts that experimented with metal detectors for a short time found them a bit frustrating.And kids all over the world are getting guns from all these places but nobody is doing anything about it. It is very easy for someone to get a handgun. If we could stop the selling of drugs just by maybe 20% or even 15%, there would be a whole less violence due to the lack of money. So nobody would be able to buy a weapon. Teachers are in as much danger of being killed or attacked, as kids are at school. The recent examples are the series of shooting which took place in 1998 started with Columbine High School in colorado. The risk of a teacher being attacked by a student has doubled since 1956. This is a lot considering that you don't hear about too many teacher attacks on the news but you do hear about teens being killed. Approximately 100 teachers have been assaulted annually in the past four school years . Also last year in about six incidents which highlighted the newspapers about 25 students along with 5 teacher who were killed due to high school shootings. The big problem involving violence is how we can stop the violence from spreading and increasing.